Induction of a proteinase inhibitor II-class gene by auxin in tomato roots

A cDNA clone corresponding to a transcript that was induced by auxin in tomato seedling roots encodes a 223 amino acid protein belonging to the proteinase inhibitor II family of tomato and potato. The TR8 open reading frame has an N-terminal region with characteristics of a signal peptide and three...

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Bibliographic Details
Published in:Plant molecular biology 1993-12, Vol.23 (5), p.1005-1014
Main Authors: Taylor, B.H. (Texas A and M Univ., College Station, TX (USA). Dept. of Biology), Young, R.J, Scheuring, C.F
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
AUXINAS
AUXINE
AUXINS
Base Sequence
Biological and medical sciences
Cloning, Molecular
Consensus Sequence
DNA
DNA, Complementary - genetics
ENZYME INHIBITORS
Fundamental and applied biological sciences. Psychology
GENE
Gene expression
Gene Expression Regulation - drug effects
GENES
Genes, Plant
Indoleacetic Acids - pharmacology
INHIBIDORES DE ENZIMAS
INHIBITEUR D'ENZYME
LYCOPERSICON ESCULENTUM
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Plant Proteins - genetics
PLANTULAS
PLANTULE
Polymorphism, Restriction Fragment Length
PROTEASAS
PROTEASE
Protease Inhibitors - metabolism
PROTEASES
RACINE
RAICES
ROOTS
SEEDLINGS
Sequence Alignment
Sequence Homology, Amino Acid
Space life sciences
Tissue Distribution
Vegetables - genetics
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Description
Summary:A cDNA clone corresponding to a transcript that was induced by auxin in tomato seedling roots encodes a 223 amino acid protein belonging to the proteinase inhibitor II family of tomato and potato. The TR8 open reading frame has an N-terminal region with characteristics of a signal peptide and three copies of a 64 amino acid segment that is also present in multiple copies in other PI-II family members. Comparison to other serine proteinase inhibitors indicates that all three domains are trypsin-specific. Transcripts homologous to TR8 increased in abundance within 24 h of auxin treatment and continued to increase through 72 h, at which point the induction was approximately ten-fold. Auxin induction was observed in roots and hypocotyls, but not in cotyledons or epicotyls. Induction may therefore be correlated with lateral and adventitious root initiation, which occurs over a similar time frame in the same tissues. RFLPs detected with the TR8 probe were mapped to the long arm of chromosome 11.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00021815