Expression of Plasmodium falciparum Circumsporozoite Proteins in Escherichia coli for Potential Use in a Human Malaria Vaccine

The circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum may be the most promising target for the development of a malaria vaccine. In this study, proteins composed of 16, 32, or 48 tandem copies of a tetrapeptide repeating sequence found in the CS protein were efficient...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1985-05, Vol.228 (4702), p.958-962
Main Authors: Young, James F., Hockmeyer, Wayne T., Gross, Mitchell, Ballou, W. Ripley, Wirtz, Robert A., Trosper, James H., Beaudoin, Richard L., Hollingdale, Michael R., Miller, Louis H., Diggs, Carter L., Rosenberg, Martin
Format: Article
Language:English
Subjects:
Aluminum
Amino acids
Animals
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antibodies
Antibody Formation
Antigens
Antigens, Surface - genetics
Antigens, Surface - immunology
Antiparasitic agents
Biological and medical sciences
Biotechnology
Carcinoma, Hepatocellular
Care and treatment
Cell Line
Cloning, Molecular
Cross Reactions
DNA, Recombinant
Escherichia coli
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
gene expression
genes
Health. Pharmaceutical industry
Human protozoal diseases
Humans
Hydroxides
Immunodominant epitopes
Industrial applications and implications. Economical aspects
Infectious diseases
Liver Neoplasms
Malaria
Malaria - prevention & control
Medical sciences
Mice
Parasitic diseases
Parasitological research
Pharmacology. Drug treatments
Plasmodium - immunology
Plasmodium falciparum
Plasmodium falciparum - genetics
Plasmodium falciparum - immunology
Plasmodium falciparum - physiology
porozoites
Production of active biomolecules
proteins
Protozoal diseases
Protozoan Proteins
Reactivity
Sporozoites
Tropical medicine
Vaccination
Vaccines
Vaccines - immunology
Vaccins
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Description
Summary:The circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum may be the most promising target for the development of a malaria vaccine. In this study, proteins composed of 16, 32, or 48 tandem copies of a tetrapeptide repeating sequence found in the CS protein were efficiently expressed in the bacterium Escherichia coli. When injected into mice, these recombinant products resulted in the production of high titers of antibodies that reacted with the authentic CS protein on live sporozoites and blocked sporozoite invasion of human hepatoma cells in vitro. These CS protein derivatives are therefore candidates for a human malaria vaccine.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2988125