Antifungal Susceptibility Testing of Yeasts: A Brief Overview

Antifungal susceptibility testing of yeasts has lacked reproducibility because of the absence of universally accepted guidelines. The major sources of variation in susceptibility testing have been attributed to the choice of medium and pH, inoculum preparation and size, incubation temperature and ti...

Full description

Saved in:
Bibliographic Details
Published in:Clinical infectious diseases 1993-11, Vol.17 (Supplement-2), p.S494-S500
Main Authors: Sheehan, Daniel J., Espinel-Ingroff, Ana, Moore, Lynn Steele, Webb, C. Douglas
Format: Article
Language:English
Subjects:
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antifungal agents
Antifungal Agents - pharmacology
Antifungals
Azoles
Biological and medical sciences
Candida
Fungi
Guidelines as Topic
Health outcomes
Imidazoles
Infections
Inoculum
Management of Fungal Infections: Part 2
Medical sciences
Microbial Sensitivity Tests
Pharmacology. Drug treatments
Triazoles
Yeasts
Yeasts - drug effects
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Antifungal susceptibility testing of yeasts has lacked reproducibility because of the absence of universally accepted guidelines. The major sources of variation in susceptibility testing have been attributed to the choice of medium and pH, inoculum preparation and size, incubation temperature and time, and end-point criteria. These parameters have been addressed by the National Committee for Clinical Laboratory Standards (NCCLS) Subcommittee on Antifungal Susceptibility Testing, and proposed guidelines have recently been published. Although major advances in antifungal susceptibility testing have been achieved, particularly through the efforts of the NCCLS subcommittee, the results of studies to date do not support a correlation between the results of in vitro susceptibility testing and in vivo response. Routine antifungal susceptibility testing of yeasts should therefore be discouraged until the methodology is standardized such that it predicts clinical outcome.
ISSN:1058-4838
1537-6591
DOI:10.1093/clinids/17.Supplement_2.S494