Primary structure of the heterosaccharide of the surface glycoprotein of Methanothermus fervidus

The outer surface of the cells of the hyperthermophile Methanothermus fervidus is covered by crystalline glycoprotein subunits (S-layer). From the purified S-layer glycoprotein, a heterosaccharide was isolated. The heterosaccharide consists of D-3-O-methylmannose, D-mannose, and D-N-acetylgalactosam...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1993-12, Vol.268 (36), p.26821-26826
Main Authors: Kärcher, U, Schröder, H, Haslinger, E, Allmaier, G, Schreiner, R, Wieland, F, Haselbeck, A, König, H
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carbohydrate Sequence
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Fundamental and applied biological sciences. Psychology
Glycoproteins
Magnetic Resonance Spectroscopy
Methanobacteriales - chemistry
Methanothermus fervidus
Molecular Sequence Data
Oligosaccharides - chemistry
Oligosaccharides - isolation & purification
Polysaccharides - analysis
Proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The outer surface of the cells of the hyperthermophile Methanothermus fervidus is covered by crystalline glycoprotein subunits (S-layer). From the purified S-layer glycoprotein, a heterosaccharide was isolated. The heterosaccharide consists of D-3-O-methylmannose, D-mannose, and D-N-acetylgalactosamine in a molar ratio of 2:3:1 corresponding to a relative molecular mass of 1061.83 Da. 3-O-methylmannose could be partly replaced by 3-O-methylglucose. The primary structure of the glycan was revealed by methylation analysis, by plasma desorption mass spectrometry, and by high field NMR spectroscopy. The purified heterosaccharide is linked via N-acetylgalactosamine to an asparagine residue of the peptide moiety. The following structure is proposed for the heterosaccharide: alpha-D-3-O-MetManp-(1-->6)-alpha-D-3-O-MetManp-((1-->2)-alp ha-D-Manp)3-(1-->4) - D-GalNAc.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)74185-4