Production of Monoclonal Anti-Idiotype Antibodies Which Mimic an M-Like Protein of Streptococcus equi

• Published in: MICROBIOLOGY and IMMUNOLOGY 1993, Vol.37(9), pp.737-742
• Main Authors: Jean-Francois, M. J. Bernadette, Poskitt, David C., MacDonald, Lisa M., Turnbull, Sally J., Yasmeen, Dilruba
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Anti-Idiotypic; Antibodies, Bacterial; Antibodies, immunoglobulins; Antibodies, Monoclonal; Antibody Specificity; Antigen-Antibody Reactions; Antigens, Bacterial - immunology; Bacterial Outer Membrane Proteins; Bacterial Proteins - immunology; Biological and medical sciences; Carrier Proteins; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Mice; Molecular immunology; Monoclonal anti-idiotype; Monoclonal antibodies; Rats; Streptococcus - immunology; Streptococcus equi
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1993.tb01699.x

Rat monoclonal anti-idiotype antibodies (mAb2) were raised against two mouse monoclonal antibodies (mAb1), 1D10 and 2A6, with specificity for the M-like protein of Streptococcus equi. The capacity of the mAb2 to inhibit the binding between the corresponding mouse mAb1 against which the mAb2 were raised and the M-like protein was investigated in an inhibition EIA. One of the ten mAb2 examined, namely 5D1 (anti-mAb1 1D10), was able to inhibit this binding. The mAb2 5D1 bound to the mAb1 1D10 in such a way as to completely inhibit the subsequent binding of the M-like protein antigen to the paratope of the mAb1 1D10. The mAb2 5D1 is likely to represent a true image of the M-like protein antigen and may thus be described as an Ab2β anti-idiotype antibody.