A housekeeping type promoter, located in the 3' region of the Duchenne muscular dystrophy gene, controls the expression of Dp71, a major product of the gene

The 70.8 kDa protein product of the distal part of the giant Duchenne muscular dystrophy (DMD) gene, Dp71, is expressed in many cell types and tissues. Anchored PCR, primer extension and functional analysis of transfected constructs were used to determine the 5' end of the mRNA and characterize...

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Bibliographic Details
Published in:Human molecular genetics 1993-11, Vol.2 (11), p.1883-1888
Main Authors: Lederfein, Doron, Yaffe, David, Nudel, Uri
Format: Article
Language:English
Subjects:
Animals
Base Composition
Base Sequence
Biological and medical sciences
Brain - metabolism
Carcinoma, Hepatocellular
Chloramphenicol O-Acetyltransferase - metabolism
Chromosome Mapping
Cloning, Molecular
Dp71 gene
Duchenne's muscular dystrophy
dystrophin
Dystrophin - analogs & derivatives
Dystrophin - genetics
Exons
Fundamental and applied biological sciences. Psychology
gene expression
gene products
genes
Humans
Liver Neoplasms
man
Mice
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Muscles - metabolism
Muscular Dystrophy, Animal - genetics
Neuroblastoma
Polymerase Chain Reaction
Promoter Regions, Genetic
promoters
Repetitive Sequences, Nucleic Acid
Restriction Mapping
RNA, Messenger - biosynthesis
RNA, Messenger - metabolism
Sequence Homology, Nucleic Acid
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
Transfection
Tumor Cells, Cultured
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Description
Summary:The 70.8 kDa protein product of the distal part of the giant Duchenne muscular dystrophy (DMD) gene, Dp71, is expressed in many cell types and tissues. Anchored PCR, primer extension and functional analysis of transfected constructs were used to determine the 5' end of the mRNA and characterize the promoter of this major DMD gene product. The 5' untranslated region (5'UTR) of Dp71 is transcribed from a single exon; the promoter does not contain a TATA box, and has a very high GC content and several potential Sp1 binding sites. It is located more than 2000 kb 3' to the muscle and brain type dystrophin promoters and only 150 kb from the 3' end of the gene, suggesting that in most DMD patients the expression of Dp71 Is unaffected.
ISSN:0964-6906
1460-2083
DOI:10.1093/hmg/2.11.1883