NADPH‐dependent generation of H2O2 in a thyroid particulate fraction requires Ca2

The action of Ca2+ on H2O2 formation catalyzed by an NADPH‐dependent H2O2‐generating system was studied in a thyroid particulate fraction. It was shown that H2O2 formation was inhibited by EGTA and restored in the presence of micromolar concentrations of free Ca2+, and that Mg2+ was unable to restor...

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Bibliographic Details
Published in:FEBS letters 1985-07, Vol.186 (1), p.107-110
Main Authors: Deme, Danielle, Virion, Alain, Hammou, Néfissa Ait, Pommier, Jacques
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Ca2
Calcium - pharmacology
Egtazic Acid - pharmacology
Fundamental and applied biological sciences. Psychology
Hormones. Regulation
Hydrogen Peroxide - metabolism
Hydroperoxide production
Magnesium - pharmacology
NADP - pharmacology
NADPH
Swine
Temperature
Thyroid
Thyroid Gland - drug effects
Thyroid Gland - metabolism
Thyroid. Parathyroid. Ultimobranchial body
Thyrotropin - pharmacology
Vertebrates: endocrinology
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Summary:The action of Ca2+ on H2O2 formation catalyzed by an NADPH‐dependent H2O2‐generating system was studied in a thyroid particulate fraction. It was shown that H2O2 formation was inhibited by EGTA and restored in the presence of micromolar concentrations of free Ca2+, and that Mg2+ was unable to restore the activity of the EGTA‐inhibited particulate fraction. Consequently, NADPH‐dependent H2O2 formation requires Ca2+, and the Ca2+ stimulation of this process is specific and reversible.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(85)81349-1