Chloroquine impairs the interferon-induced antiviral state without affecting the 2‘,5‘-oligoadenylate synthetase

Chloroquine, a weak base which raises the pH in acidic cellular compartments such as lysosomes and endosomes, counteracts the induction by interferon of the antiviral state but not that of the 2',5'-oligoadenylate synthetase in three different types of cell lines (MDBK, WISH, and L929). Ac...

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Bibliographic Details
Published in:The Journal of biological chemistry 1985-07, Vol.260 (13), p.7960-7964
Main Authors: Chelbi-Alix, M K, Thang, M N
Format: Article
Language:English
Subjects:
2',5'-Oligoadenylate Synthetase - metabolism
Analysis of the immune response. Humoral and cellular immunity
Animals
Antiviral Agents
Biological and medical sciences
Cattle
Cell Line
Chloroquine - pharmacology
Female
Fibroblasts - drug effects
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunobiology
Interferon Type I - pharmacology
Interferons - pharmacology
Kidney - drug effects
Mice
Miscellaneous
Pregnancy
Regulatory factors and their cellular receptors
Vesicular stomatitis Indiana virus - drug effects
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Summary:Chloroquine, a weak base which raises the pH in acidic cellular compartments such as lysosomes and endosomes, counteracts the induction by interferon of the antiviral state but not that of the 2',5'-oligoadenylate synthetase in three different types of cell lines (MDBK, WISH, and L929). Active interferon is recovered in crude extracts of cells which have been treated with interferon and chloroquine together, but not in extracts of cells treated with interferon alone, indicating that chloroquine has inhibited the intralysosomal proteolysis of interferon. A low pH-dependent event in the intracellular fate of interferon (perhaps its intralysosomal degradation) is, therefore, necessary for the establishment of the antiviral state but not for the induction of the 2',5'-oligoadenylate synthetase.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)39546-7