Long-Term Serial Cultivation and Growth Requirements for Human Umbilical Vein Endothelial Cells

Human umbilical vein endothelial cells (HUV-EC) grew rapidly in vitro in medium supplemented with epidermal growth factor, fetal bovine serum (FBS) and human diploid fibroblast-conditioned medium. The effect of FBS could be replaced partially by bovine serum albumin, cholesterol, and vitamin E, and...

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Bibliographic Details
Published in:In Vitro Cellular & Developmental Biology 1985-03, Vol.21 (3), p.181-188
Main Authors: Kan, Mikio, Miwa Kato, Isao Yamane
Format: Article
Language:English
Subjects:
Cell Division - drug effects
Cell growth
Cell Survival
Cells, Cultured
Culture Media
Cultured cells
Dialysis solutions
Diploidy
Endothelial cells
Endothelial growth factors
Endothelium - cytology
Epidermal Growth Factor - pharmacology
Fetal Blood - physiology
Fibroblast growth factors
Fibroblasts
Fibroblasts - cytology
Fibroblasts - metabolism
Human growth
Human umbilical vein endothelial cells
Humans
Mitogens - pharmacology
Time Factors
Umbilical Veins - cytology
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Summary:Human umbilical vein endothelial cells (HUV-EC) grew rapidly in vitro in medium supplemented with epidermal growth factor, fetal bovine serum (FBS) and human diploid fibroblast-conditioned medium. The effect of FBS could be replaced partially by bovine serum albumin, cholesterol, and vitamin E, and completely by further addition of serum dialysate or refeeding every other day. Among these components, fibroblast-conditioned medium is essential for HUV-EC growth. The HUV-EC were cultured serially for over 50 population doublings in the 10% FBS containing fibroblast-conditioned medium and for over 40 population doublings in the serum-free medium. Mitogenic factor(s) present in the medium conditioned by fibroblasts may be related to endothelial cell growth factor and play an important role in angiogenesis and regeneration of vascular endothelium in vitro.
ISSN:0883-8364
0073-5655
1475-2689
DOI:10.1007/BF02621356