Production of transgenic rabbits, sheep and pigs by microinjection

• Published in: Nature (London) 1985-06, Vol.315 (6021), p.680-683
• Main Authors: Hammer, Robert E, Pursel, Vernon G, Rexroad, Caird E, Wall, Robert J, Bolt, Douglas J, Ebert, Karl M, Palmiter, Richard D, Brinster, Ralph L
• Format: Article
• Language: English
• Subjects: Animals; Gene Expression Regulation; Genetic Techniques; Growth Hormone - genetics; Humanities and Social Sciences; Humans; letter; Metallothionein - genetics; Microinjections; multidisciplinary; Mutation; Promoter Regions, Genetic; Rabbits - genetics; Science; Science (multidisciplinary); Sheep - genetics; Swine - genetics
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/315680a0

Direct microinjection has been used to introduce foreign DNA into a number of terminally differentiated cell types as well as embryos of several species including sea urchin 1 , Candida elegans 2 , Xenopus 3 , Drosophila 4,5 and mice 6–11 . Various genes have been successfully introduced into mice including constructs consisting of the mouse metallothionein-I (MT) promoter/regulator region fused to either the rat or human growth hormone (hGH) structural genes. Transgenic mice harbouring such genes commonly exhibit high, metal-inducible levels of the fusion messenger RNA in several organs, substantial quantities of the foreign growth hormone in serum and enhanced growth 12,13 . In addition, the gene is stably incorporated into the germ line, making the phenotype heritable. Because of the scientific importance and potential economic value of transgenic livestock containing foreign genes, we initiated studies on large animals by microinjecting the fusion gene, MT – hGH 13 , into the pronuclei or nuclei of eggs from superovulated rabbits, sheep and pigs. We report here integration of the gene in all three species and expression of the gene in transgenic rabbits and pigs.