Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs

Two distinct but distantly related complementary DNAs encoding proteins sharing human interleukin-1 (IL-1) activity (termed IL-lα and IL-1β), were isolated from a macrophage cDNA library. The primary translation products of the genes are 271 and 269 amino acids long, although expression in Escherich...

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Bibliographic Details
Published in:Nature (London) 1985-06, Vol.315 (6021), p.641-647
Main Authors: March, Carl J, Mosley, Bruce, Larsen, Alf, Cerretti, Douglas Pat, Braedt, Gary, Price, Virginia, Gillis, Steven, Henney, Christopher S, Kronheim, Shirley R, Grabstein, Kenneth, Conlon, Paul J, Hopp, Thomas P, Cosman, David
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA Restriction Enzymes
DNA, Circular - genetics
Gene Expression Regulation
Humanities and Social Sciences
Humans
Interleukin-1 - analysis
Interleukin-1 - genetics
multidisciplinary
Plasmids
RNA, Messenger - isolation & purification
Science
Science (multidisciplinary)
Xenopus
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Summary:Two distinct but distantly related complementary DNAs encoding proteins sharing human interleukin-1 (IL-1) activity (termed IL-lα and IL-1β), were isolated from a macrophage cDNA library. The primary translation products of the genes are 271 and 269 amino acids long, although expression in Escherichia coli of the carboxy-terminal 159 and 153 amino acids produces IL-1 biological activity.
ISSN:0028-0836
1476-4687
DOI:10.1038/315641a0