Ultrastructural observations on the transvascular route of protein removal in vasogenic brain edema

Micro-blood vessels (MBVs), located in the area of edema, were studied in cat brain at various time intervals (1 h, 24 h, 7 days) after cold-lesion injury. Both cold-injured and adjacent gyri were examined for blood-brain barrier (BBB) permeability to i. v. injected horseradish peroxidase (HRP) with...

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Bibliographic Details
Published in:Acta neuropathologica 1985, Vol.66 (4), p.265-273
Main Authors: VORBRODT, A. W, LOSSINSKY, A. S, WISNIEWSKI, H. M, SUZUKI, R, YAMAGUCHI, T, MASAOKA, H, KLAIZO, I
Format: Article
Language:English
Subjects:
Alkaline Phosphatase
Animals
Biological and medical sciences
Blood Proteins - metabolism
Blood-Brain Barrier
Brain Edema - pathology
Cats
Female
Ferritins
Horseradish Peroxidase
Male
Medical sciences
Microcirculation - ultrastructure
Nervous system involvement in other diseases. Miscellaneous
Neurology
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Summary:Micro-blood vessels (MBVs), located in the area of edema, were studied in cat brain at various time intervals (1 h, 24 h, 7 days) after cold-lesion injury. Both cold-injured and adjacent gyri were examined for blood-brain barrier (BBB) permeability to i. v. injected horseradish peroxidase (HRP) with circulation times of 40 min and 24 h. Evans blue (EB) was used as a tracer for gross evaluation of the extension of brain edema. Localization of alkaline phosphatase (AP) and binding of cationized ferritin (CF), considered as a marker of anionic sites, were also studied ultrastructurally. Twenty-four hours after cold injury, the extravasated edema fluid, outlined by EB tracer, was observed to be spreading through the white matter (WM) into the adjacent gyrus. At this time, numerous, larger than capillary MBVs, presumably arterioles and venules located in the edematous WM, showed accumulations of HRP injected at the time of the operation, in the basement membrane, in abluminal pits, and in numerous pinocytotic vesicles and vacuoles of endothelial cells (ECs). The animals killed after 24 h with 40 min HRP circulation showed extravasation of HRP tracer in a zone underlying the necrotic cold injury lesion. On the other hand, there was no evidence of an abnormal HRP leakage in the further removed areas of edema in the WM, particularly in the adjacent gyrus.
ISSN:0001-6322
1432-0533
DOI:10.1007/BF00690958