Automated process monitoring of monoclonal antibody production

Antifibronectin, monoclonal antibody was monitored through 52 h of production. Samples were automatically drawn from a bioreactor into the injection valve of an HPLC system without prior sample preparation. The hybridoma cell line was nonadherent, so whole cells were injected directly onto the perfu...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1993-12, Vol.65 (23), p.3363-3367
Main Authors: Paliwal, Sandeep K, Nadler, Timothy K, Wang, Daniel I. C, Regnier, Fred E
Format: Article
Language:English
Subjects:
Animal cells
Animals
Antibodies, Monoclonal - biosynthesis
Biochemistry
Biological and medical sciences
Biotechnology
Cell hybridization, cell fusion, hybridomas
Chromatography, High Pressure Liquid
Eukaryotic cell cultures
Fibronectins - immunology
Fundamental and applied biological sciences. Psychology
Hybridomas
Hydrogen-Ion Concentration
Immunoglobulin G - biosynthesis
Methods. Procedures. Technologies
Proteins
Spectrophotometry, Ultraviolet
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Description
Summary:Antifibronectin, monoclonal antibody was monitored through 52 h of production. Samples were automatically drawn from a bioreactor into the injection valve of an HPLC system without prior sample preparation. The hybridoma cell line was nonadherent, so whole cells were injected directly onto the perfusable protein A affinity column. There was only a modest column back pressure (ca. 1700 psi at a linear flow rate of 1.5 cm/s) after over 75 injections over the 52-h experiment. These experiments demonstrate the utility of high-speed chromatography for rapid process monitoring.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac00071a005