Self-diploidization in Saccharomyces cerevisiae kar2 heterokaryons

Zygotes isolated by micromanipulation from crosses of Saccharomyces cerevisiae strains, one of which carries a kar mutation, give rise most frequently to cytoductant colonies showing the nuclear constitution of either one of the two haploid parental strains. In crosses of kar2-1 strains to wild-type...

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Bibliographic Details
Published in:Current genetics 1993-11, Vol.24 (5), p.369-372
Main Authors: POLAINA, J, ADAM, A. C, DEL CASTILLO, L
Format: Article
Language:English
Subjects:
accouplement
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
copula
copulation
Crosses, Genetic
diploidia
diploidie
Diploidy
Fundamental and applied biological sciences. Psychology
Genes, Fungal
Genetics of eukaryotes. Biological and molecular evolution
Genotype
Karyotyping
mutant
mutantes
mutants
Mutation
Phenotype
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Thallophyta, bryophyta
Vegetals
Zygote
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Summary:Zygotes isolated by micromanipulation from crosses of Saccharomyces cerevisiae strains, one of which carries a kar mutation, give rise most frequently to cytoductant colonies showing the nuclear constitution of either one of the two haploid parental strains. In crosses of kar2-1 strains to wild-type, about 10% of the cytoductants of both mating types are homozygous autodiploids. There is evidence indicating that self-diploidization occurs by fusion between sibling nuclei in the heterokaryotic zygote. Here this phenomenon is described and it is proposed to take advantage of it for the construction of genotypically-defined diploids able to mate, and of polyploid strains, which are useful tools in genetic and cytological studies.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00351842