Polymyxin B neutralizes bacteria-released endotoxin and improves the quality of boar sperm during liquid storage and cryopreservation

Gram negative bacteria are the predominant type detected in boar semen. Since these bacteria release lipopolysaccharide (LPS), and because polymyxin B (PMB) neutralizes LPS activity, the objective was to improve techniques for long-term storage of boar sperm by testing the beneficial effects of PMB....

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Bibliographic Details
Published in:Theriogenology 2010-12, Vol.74 (9), p.1691-1700
Main Authors: Okazaki, Tetsuji, Mihara, Toshihiro, Fujita, Youko, Yoshida, Shuji, Teshima, Hisanori, Shimada, Masayuki
Format: Article
Language:English
Subjects:
additives
Animals
antibacterial properties
apoptosis
Apoptosis - drug effects
Artificial insemination
Bacterial infection
benzylpenicillin
boars
cold storage
cryopreservation
Cryopreservation - veterinary
ejaculation
Endotoxin
endotoxins
Female
Gram-negative bacteria
immunity
Innate immune function
Insemination, Artificial - veterinary
lipopolysaccharides
Lipopolysaccharides - chemistry
Lipopolysaccharides - toxicity
Male
male fertility
microbiological quality
neutralization
penicillins
polymyxin B
Polymyxin B - pharmacology
Protective Agents - pharmacology
Seminal plasma
sperm motility
Sperm Motility - drug effects
spermatozoa
Spermatozoa - drug effects
Spermatozoa - microbiology
storage quality
storage time
Swine - microbiology
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Summary:Gram negative bacteria are the predominant type detected in boar semen. Since these bacteria release lipopolysaccharide (LPS), and because polymyxin B (PMB) neutralizes LPS activity, the objective was to improve techniques for long-term storage of boar sperm by testing the beneficial effects of PMB. In the present study, LPS bound directly to the head region of sperm, decreased sperm motility, and induced sperm apoptosis. The addition of 100 μg/mL PMB suppressed LPS binding activity and blocked the negative effects of LPS on sperm quality. Additionally, when PMB treatment was combined with penicillin G (PenG), sperm motility was increased after 10 d of liquid storage or in frozen-thawed sperm (P
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2010.05.019