Reversed-phase HPLC of peptides: Assessing column and solvent selectivity on standard, polar-embedded and polar endcapped columns

We desired to evaluate the chromatographic selectivity for peptides of silica‐based RP high‐performance liquid chromatography stationary phases with various modifications (polar embedding and polar endcapping on C18 columns; ether‐linked phenyl column with polar endcapping) compared with n‐alkyl (C1...

Full description

Saved in:
Bibliographic Details
Published in:Journal of separation science 2010-10, Vol.33 (19), p.3005-3021
Main Authors: Mant, Colin T., Cepeniene, Dziuleta, Hodges, Robert S.
Format: Article
Language:English
Subjects:
ACN
Chromatography, High Pressure Liquid - methods
Hydrophobic and Hydrophilic Interactions
Methanol
Methanol - chemistry
Peptides
Peptides - chemistry
RP-HPLC
Silicon Dioxide - chemistry
Solvents - chemistry
Stationary phases
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We desired to evaluate the chromatographic selectivity for peptides of silica‐based RP high‐performance liquid chromatography stationary phases with various modifications (polar embedding and polar endcapping on C18 columns; ether‐linked phenyl column with polar endcapping) compared with n‐alkyl (C18, C8) and aromatic phenylhexyl columns. Thus, we have designed and synthesized two series of synthetic peptide standards with the sequence Gly–Gly–Leu–Gly–Gly–Ala–Leu–Gly–X–Leu–Lys–Lys–amide, where the N‐terminal either contains a free α‐amino group (AmC series) or is Nα‐acetylated (AcC series) and where position X is substituted by Gly, Ala, Val, Ile, Phe or Tyr. These represent series of peptides with single substitutions of n‐alkyl (Gly
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201000518