The developmental changes of mRNA levels for a cerebellar protein (spot 35 protein) in rat brains

Our previous papers described a protein called spot 35 found in the cerebellar cytosol of adult rats by two-dimensional gel electrophoresis and localized in the Purkinje cells by immunohistochemical methods. Here we describe the biosynthesis of this spot 35 protein using a reticulocyte lysate cell-f...

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Bibliographic Details
Published in:FEBS letters 1985-08, Vol.188 (1), p.127-130
Main Authors: Yamakuni, T., Araki, K., Takahashi, Y.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain - embryology
Brain - growth & development
Brain - metabolism
Brain development
Calbindin 1
Calbindins
Calcium-Binding Proteins
Cell-Free System
Cerebellar protein
Cerebellum - metabolism
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Immunodiffusion
Immunosorbent Techniques
Molecular and cellular biology
Molecular genetics
mRNA
Nerve Tissue Proteins - biosynthesis
Protein Biosynthesis
Rats
Reticulocytes - metabolism
RNA, Messenger - metabolism
S100 Calcium Binding Protein G
Translation
Translation. Translation factors. Protein processing
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Summary:Our previous papers described a protein called spot 35 found in the cerebellar cytosol of adult rats by two-dimensional gel electrophoresis and localized in the Purkinje cells by immunohistochemical methods. Here we describe the biosynthesis of this spot 35 protein using a reticulocyte lysate cell-free system containing rat cerebellar mRNA. The developmental changes of mRNA-dependent protein biosynthesis were also examined. During postnatal 10–30 days, a rapid increase of mRNA levels for spot 35 protein was observed. The application of the new 45Ca-binding assay procedure revealed that this protein is a Ca-binding protein.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(85)80888-7