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The developmental changes of mRNA levels for a cerebellar protein (spot 35 protein) in rat brains
Our previous papers described a protein called spot 35 found in the cerebellar cytosol of adult rats by two-dimensional gel electrophoresis and localized in the Purkinje cells by immunohistochemical methods. Here we describe the biosynthesis of this spot 35 protein using a reticulocyte lysate cell-f...
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| Published in: | FEBS letters 1985-08, Vol.188 (1), p.127-130 |
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| Main Authors: | , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c5077-c43225de1f071b1a223baa9cf243cbba7f92788426bc4da2b4fa3f10a8ff53363 |
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| cites | cdi_FETCH-LOGICAL-c5077-c43225de1f071b1a223baa9cf243cbba7f92788426bc4da2b4fa3f10a8ff53363 |
| container_end_page | 130 |
| container_issue | 1 |
| container_start_page | 127 |
| container_title | FEBS letters |
| container_volume | 188 |
| creator | Yamakuni, T. Araki, K. Takahashi, Y. |
| description | Our previous papers described a protein called spot 35 found in the cerebellar cytosol of adult rats by two-dimensional gel electrophoresis and localized in the Purkinje cells by immunohistochemical methods. Here we describe the biosynthesis of this spot 35 protein using a reticulocyte lysate cell-free system containing rat cerebellar mRNA. The developmental changes of mRNA-dependent protein biosynthesis were also examined. During postnatal 10–30 days, a rapid increase of mRNA levels for spot 35 protein was observed. The application of the new
45Ca-binding assay procedure revealed that this protein is a Ca-binding protein. |
| doi_str_mv | 10.1016/0014-5793(85)80888-7 |
| format | article |
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45Ca-binding assay procedure revealed that this protein is a Ca-binding protein.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain - embryology</subject><subject>Brain - growth & development</subject><subject>Brain - metabolism</subject><subject>Brain development</subject><subject>Calbindin 1</subject><subject>Calbindins</subject><subject>Calcium-Binding Proteins</subject><subject>Cell-Free System</subject><subject>Cerebellar protein</subject><subject>Cerebellum - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. 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Here we describe the biosynthesis of this spot 35 protein using a reticulocyte lysate cell-free system containing rat cerebellar mRNA. The developmental changes of mRNA-dependent protein biosynthesis were also examined. During postnatal 10–30 days, a rapid increase of mRNA levels for spot 35 protein was observed. The application of the new
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| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1985-08, Vol.188 (1), p.127-130 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76212911 |
| source | ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Brain - embryology Brain - growth & development Brain - metabolism Brain development Calbindin 1 Calbindins Calcium-Binding Proteins Cell-Free System Cerebellar protein Cerebellum - metabolism Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Immunodiffusion Immunosorbent Techniques Molecular and cellular biology Molecular genetics mRNA Nerve Tissue Proteins - biosynthesis Protein Biosynthesis Rats Reticulocytes - metabolism RNA, Messenger - metabolism S100 Calcium Binding Protein G Translation Translation. Translation factors. Protein processing |
| title | The developmental changes of mRNA levels for a cerebellar protein (spot 35 protein) in rat brains |
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