Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid

A cDNA library from rat brain was constructed in pBR322 and screened with a 14-mer mixed oligonucleotide probe based on residues 231–235 of bovine proteolipid (PLP). A positive clone was isolated: it contained a 1334-base-pair cDNA insert and was subjected to DNA sequence analysis. The cDNA encoded...

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Bibliographic Details
Published in:FEBS letters 1985-08, Vol.188 (1), p.33-36
Main Authors: Dautigny, André, Alliel, Patrick M., d'Auriol, Luc, Dinh, Daniéle Pham, Nussbaum, Jean-Louis, Galibert, Francis, Joliès, Pierre
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Brain Chemistry
cDNA sequence
Cloning, Molecular
DNA - genetics
DNA, Recombinant
Fundamental and applied biological sciences. Psychology
Genes. Genome
Molecular and cellular biology
Molecular genetics
Myelin
Myelin Proteins - genetics
Myelin Proteolipid Protein
Nucleic Acid Hybridization
Oligonucleotide probe
Plasmids
Proteolipid
Rat brain
Rats
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Summary:A cDNA library from rat brain was constructed in pBR322 and screened with a 14-mer mixed oligonucleotide probe based on residues 231–235 of bovine proteolipid (PLP). A positive clone was isolated: it contained a 1334-base-pair cDNA insert and was subjected to DNA sequence analysis. The cDNA encoded information for the 276 amino acids of rat PLP. Comparison with bovine PLP sequence showed a complete amino acid sequence homology except for 4 amino acid residues.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(85)80869-3