Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid

A cDNA library from rat brain was constructed in pBR322 and screened with a 14-mer mixed oligonucleotide probe based on residues 231–235 of bovine proteolipid (PLP). A positive clone was isolated: it contained a 1334-base-pair cDNA insert and was subjected to DNA sequence analysis. The cDNA encoded...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1985-08, Vol.188 (1), p.33-36
Main Authors: Dautigny, André, Alliel, Patrick M., d'Auriol, Luc, Dinh, Daniéle Pham, Nussbaum, Jean-Louis, Galibert, Francis, Joliès, Pierre
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Brain Chemistry
cDNA sequence
Cloning, Molecular
DNA - genetics
DNA, Recombinant
Fundamental and applied biological sciences. Psychology
Genes. Genome
Molecular and cellular biology
Molecular genetics
Myelin
Myelin Proteins - genetics
Myelin Proteolipid Protein
Nucleic Acid Hybridization
Oligonucleotide probe
Plasmids
Proteolipid
Rat brain
Rats
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c5263-bc855bdeee1c05014a561ff41161037af557b0ef79a88f89d1aae0dfa13ec22d3
cites cdi_FETCH-LOGICAL-c5263-bc855bdeee1c05014a561ff41161037af557b0ef79a88f89d1aae0dfa13ec22d3
container_end_page 36
container_issue 1
container_start_page 33
container_title FEBS letters
container_volume 188
creator Dautigny, André
Alliel, Patrick M.
d'Auriol, Luc
Dinh, Daniéle Pham
Nussbaum, Jean-Louis
Galibert, Francis
Joliès, Pierre
description A cDNA library from rat brain was constructed in pBR322 and screened with a 14-mer mixed oligonucleotide probe based on residues 231–235 of bovine proteolipid (PLP). A positive clone was isolated: it contained a 1334-base-pair cDNA insert and was subjected to DNA sequence analysis. The cDNA encoded information for the 276 amino acids of rat PLP. Comparison with bovine PLP sequence showed a complete amino acid sequence homology except for 4 amino acid residues.
doi_str_mv 10.1016/0014-5793(85)80869-3
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76213563</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0014579385808693</els_id><sourcerecordid>76213563</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5263-bc855bdeee1c05014a561ff41161037af557b0ef79a88f89d1aae0dfa13ec22d3</originalsourceid><addsrcrecordid>eNqNkU1v1DAQhi0EKkvhH4DkA0JwCPgjdpxLpVK6BanABc6WY4-RkTde7KTV_nuc3dUeKaeR533mw-8g9JKS95RQ-YEQ2jai6_lbJd4pomTf8EdoRVXHG95K9RitTshT9KyU36S-Fe3P0BlrKWF9u0Lma4pg52gytjGNYfyFzejwONsIaQoOcIE_M4wWcPLYYPvp2-WeBGyTW3CfMs5mwkM2YcSbHcQatjlNkGLYBvccPfEmFnhxjOfo5_r6x9Xn5vb7zZery9vGCiZ5M1glxOAAgFoi6t5GSOp9S6mkhHfGC9ENBHzXG6W86h01BojzhnKwjDl-jt4c-tbZdeMy6U0oFmI0I6S56E4yyoXkD4K0ZYxXzyrYHkCbUykZvN7msDF5pynRywn04q9e_NVK6P0J9FL26th_HjbgTkVHz6v--qibYk302Yw2lBOmJBWSiYqtD9h9iLD7r9F6ff2RLcKSV2KfXfa5ODSC6v5dgKyLDctFXchgJ-1S-PeH_gJ_h7Wo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14223793</pqid></control><display><type>article</type><title>Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid</title><source>ScienceDirect Journals</source><creator>Dautigny, André ; Alliel, Patrick M. ; d'Auriol, Luc ; Dinh, Daniéle Pham ; Nussbaum, Jean-Louis ; Galibert, Francis ; Joliès, Pierre</creator><creatorcontrib>Dautigny, André ; Alliel, Patrick M. ; d'Auriol, Luc ; Dinh, Daniéle Pham ; Nussbaum, Jean-Louis ; Galibert, Francis ; Joliès, Pierre</creatorcontrib><description>A cDNA library from rat brain was constructed in pBR322 and screened with a 14-mer mixed oligonucleotide probe based on residues 231–235 of bovine proteolipid (PLP). A positive clone was isolated: it contained a 1334-base-pair cDNA insert and was subjected to DNA sequence analysis. The cDNA encoded information for the 276 amino acids of rat PLP. Comparison with bovine PLP sequence showed a complete amino acid sequence homology except for 4 amino acid residues.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(85)80869-3</identifier><identifier>PMID: 2410294</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Brain Chemistry ; cDNA sequence ; Cloning, Molecular ; DNA - genetics ; DNA, Recombinant ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Molecular and cellular biology ; Molecular genetics ; Myelin ; Myelin Proteins - genetics ; Myelin Proteolipid Protein ; Nucleic Acid Hybridization ; Oligonucleotide probe ; Plasmids ; Proteolipid ; Rat brain ; Rats</subject><ispartof>FEBS letters, 1985-08, Vol.188 (1), p.33-36</ispartof><rights>1985</rights><rights>FEBS Letters 188 (1985) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5263-bc855bdeee1c05014a561ff41161037af557b0ef79a88f89d1aae0dfa13ec22d3</citedby><cites>FETCH-LOGICAL-c5263-bc855bdeee1c05014a561ff41161037af557b0ef79a88f89d1aae0dfa13ec22d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579385808693$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3547,27923,27924,45779</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=8615625$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2410294$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dautigny, André</creatorcontrib><creatorcontrib>Alliel, Patrick M.</creatorcontrib><creatorcontrib>d'Auriol, Luc</creatorcontrib><creatorcontrib>Dinh, Daniéle Pham</creatorcontrib><creatorcontrib>Nussbaum, Jean-Louis</creatorcontrib><creatorcontrib>Galibert, Francis</creatorcontrib><creatorcontrib>Joliès, Pierre</creatorcontrib><title>Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>A cDNA library from rat brain was constructed in pBR322 and screened with a 14-mer mixed oligonucleotide probe based on residues 231–235 of bovine proteolipid (PLP). A positive clone was isolated: it contained a 1334-base-pair cDNA insert and was subjected to DNA sequence analysis. The cDNA encoded information for the 276 amino acids of rat PLP. Comparison with bovine PLP sequence showed a complete amino acid sequence homology except for 4 amino acid residues.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Brain Chemistry</subject><subject>cDNA sequence</subject><subject>Cloning, Molecular</subject><subject>DNA - genetics</subject><subject>DNA, Recombinant</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Myelin</subject><subject>Myelin Proteins - genetics</subject><subject>Myelin Proteolipid Protein</subject><subject>Nucleic Acid Hybridization</subject><subject>Oligonucleotide probe</subject><subject>Plasmids</subject><subject>Proteolipid</subject><subject>Rat brain</subject><subject>Rats</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNqNkU1v1DAQhi0EKkvhH4DkA0JwCPgjdpxLpVK6BanABc6WY4-RkTde7KTV_nuc3dUeKaeR533mw-8g9JKS95RQ-YEQ2jai6_lbJd4pomTf8EdoRVXHG95K9RitTshT9KyU36S-Fe3P0BlrKWF9u0Lma4pg52gytjGNYfyFzejwONsIaQoOcIE_M4wWcPLYYPvp2-WeBGyTW3CfMs5mwkM2YcSbHcQatjlNkGLYBvccPfEmFnhxjOfo5_r6x9Xn5vb7zZery9vGCiZ5M1glxOAAgFoi6t5GSOp9S6mkhHfGC9ENBHzXG6W86h01BojzhnKwjDl-jt4c-tbZdeMy6U0oFmI0I6S56E4yyoXkD4K0ZYxXzyrYHkCbUykZvN7msDF5pynRywn04q9e_NVK6P0J9FL26th_HjbgTkVHz6v--qibYk302Yw2lBOmJBWSiYqtD9h9iLD7r9F6ff2RLcKSV2KfXfa5ODSC6v5dgKyLDctFXchgJ-1S-PeH_gJ_h7Wo</recordid><startdate>19850819</startdate><enddate>19850819</enddate><creator>Dautigny, André</creator><creator>Alliel, Patrick M.</creator><creator>d'Auriol, Luc</creator><creator>Dinh, Daniéle Pham</creator><creator>Nussbaum, Jean-Louis</creator><creator>Galibert, Francis</creator><creator>Joliès, Pierre</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19850819</creationdate><title>Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid</title><author>Dautigny, André ; Alliel, Patrick M. ; d'Auriol, Luc ; Dinh, Daniéle Pham ; Nussbaum, Jean-Louis ; Galibert, Francis ; Joliès, Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5263-bc855bdeee1c05014a561ff41161037af557b0ef79a88f89d1aae0dfa13ec22d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Brain Chemistry</topic><topic>cDNA sequence</topic><topic>Cloning, Molecular</topic><topic>DNA - genetics</topic><topic>DNA, Recombinant</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Myelin</topic><topic>Myelin Proteins - genetics</topic><topic>Myelin Proteolipid Protein</topic><topic>Nucleic Acid Hybridization</topic><topic>Oligonucleotide probe</topic><topic>Plasmids</topic><topic>Proteolipid</topic><topic>Rat brain</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dautigny, André</creatorcontrib><creatorcontrib>Alliel, Patrick M.</creatorcontrib><creatorcontrib>d'Auriol, Luc</creatorcontrib><creatorcontrib>Dinh, Daniéle Pham</creatorcontrib><creatorcontrib>Nussbaum, Jean-Louis</creatorcontrib><creatorcontrib>Galibert, Francis</creatorcontrib><creatorcontrib>Joliès, Pierre</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dautigny, André</au><au>Alliel, Patrick M.</au><au>d'Auriol, Luc</au><au>Dinh, Daniéle Pham</au><au>Nussbaum, Jean-Louis</au><au>Galibert, Francis</au><au>Joliès, Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1985-08-19</date><risdate>1985</risdate><volume>188</volume><issue>1</issue><spage>33</spage><epage>36</epage><pages>33-36</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>A cDNA library from rat brain was constructed in pBR322 and screened with a 14-mer mixed oligonucleotide probe based on residues 231–235 of bovine proteolipid (PLP). A positive clone was isolated: it contained a 1334-base-pair cDNA insert and was subjected to DNA sequence analysis. The cDNA encoded information for the 276 amino acids of rat PLP. Comparison with bovine PLP sequence showed a complete amino acid sequence homology except for 4 amino acid residues.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2410294</pmid><doi>10.1016/0014-5793(85)80869-3</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0014-5793
ispartof FEBS letters, 1985-08, Vol.188 (1), p.33-36
issn 0014-5793
1873-3468
language eng
recordid cdi_proquest_miscellaneous_76213563
source ScienceDirect Journals
subjects Animals
Base Sequence
Biological and medical sciences
Brain Chemistry
cDNA sequence
Cloning, Molecular
DNA - genetics
DNA, Recombinant
Fundamental and applied biological sciences. Psychology
Genes. Genome
Molecular and cellular biology
Molecular genetics
Myelin
Myelin Proteins - genetics
Myelin Proteolipid Protein
Nucleic Acid Hybridization
Oligonucleotide probe
Plasmids
Proteolipid
Rat brain
Rats
title Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T05%3A39%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20cloning%20and%20nucleotide%20sequence%20of%20a%20cDNA%20clone%20coding%20for%20rat%20brain%20myelin%20proteolipid&rft.jtitle=FEBS%20letters&rft.au=Dautigny,%20Andr%C3%A9&rft.date=1985-08-19&rft.volume=188&rft.issue=1&rft.spage=33&rft.epage=36&rft.pages=33-36&rft.issn=0014-5793&rft.eissn=1873-3468&rft.coden=FEBLAL&rft_id=info:doi/10.1016/0014-5793(85)80869-3&rft_dat=%3Cproquest_cross%3E76213563%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c5263-bc855bdeee1c05014a561ff41161037af557b0ef79a88f89d1aae0dfa13ec22d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=14223793&rft_id=info:pmid/2410294&rfr_iscdi=true