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APPLICATION OF A TISSUE CULTURE MICROTITER TEST FOR THE DETECTION OF CYTOTOXIC AGENTS FROM NATURAL PRODUCTS
A method is described by which the growth inhibitory effects of cytotoxic compounds and fermentation broth cultures on adherent tumor cell lines can be quantitated. Cells are seeded into 96-well microtiter plates and 16 hours later the test compounds or broths are added to the wells. Cell growth is...
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| Published in: | Journal of antibiotics 1985, Vol.38(6), pp.758-766 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c5218-62066eed18bc228ab50dcfccab36eb6b4e3cb3f0e76249760c7cfde7ebd9a3753 |
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| container_end_page | 766 |
| container_issue | 6 |
| container_start_page | 758 |
| container_title | Journal of antibiotics |
| container_volume | 38 |
| creator | MIRABELLI, CHRISTOPHER K. BARTUS, HENRY BARTUS, JOAN O. L. JOHNSON, RANDALL MONG, SHAU MING SUNG, CHENG PO CROOKE, STANLEY T. |
| description | A method is described by which the growth inhibitory effects of cytotoxic compounds and fermentation broth cultures on adherent tumor cell lines can be quantitated. Cells are seeded into 96-well microtiter plates and 16 hours later the test compounds or broths are added to the wells. Cell growth is measured after three days (B16 mouse melanoma cells) or six days (HT-29, human colon carcinoma cells) by first fixing adherent cells, staining with Giemsa stain, washing away excess stain, then solubilizing stained cells with HCl. Absorbance is determined using a microELISA spectrophotometer and the data are transferred to and analyzed by a computer. The assay is rapid and reproducible and can be used to identify fermentation broths with cytotoxic components. Addition of DNA into the assay mixture (cells plus compound) inhibits the cytotoxic activities of certain DNA-reactive agents. The results of this study demonstrate the application of this assay system for primary and secondary evaluation of fermentation broths for in vitro antitumor activity. |
| doi_str_mv | 10.7164/antibiotics.38.758 |
| format | article |
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L. ; JOHNSON, RANDALL ; MONG, SHAU MING ; SUNG, CHENG PO ; CROOKE, STANLEY T.</creator><creatorcontrib>MIRABELLI, CHRISTOPHER K. ; BARTUS, HENRY ; BARTUS, JOAN O. L. ; JOHNSON, RANDALL ; MONG, SHAU MING ; SUNG, CHENG PO ; CROOKE, STANLEY T.</creatorcontrib><description>A method is described by which the growth inhibitory effects of cytotoxic compounds and fermentation broth cultures on adherent tumor cell lines can be quantitated. Cells are seeded into 96-well microtiter plates and 16 hours later the test compounds or broths are added to the wells. Cell growth is measured after three days (B16 mouse melanoma cells) or six days (HT-29, human colon carcinoma cells) by first fixing adherent cells, staining with Giemsa stain, washing away excess stain, then solubilizing stained cells with HCl. Absorbance is determined using a microELISA spectrophotometer and the data are transferred to and analyzed by a computer. The assay is rapid and reproducible and can be used to identify fermentation broths with cytotoxic components. Addition of DNA into the assay mixture (cells plus compound) inhibits the cytotoxic activities of certain DNA-reactive agents. The results of this study demonstrate the application of this assay system for primary and secondary evaluation of fermentation broths for in vitro antitumor activity.</description><identifier>ISSN: 0021-8820</identifier><identifier>EISSN: 1881-1469</identifier><identifier>DOI: 10.7164/antibiotics.38.758</identifier><identifier>PMID: 4019319</identifier><identifier>CODEN: JANTAJ</identifier><language>eng</language><publisher>Tokyo: JAPAN ANTIBIOTICS RESEARCH ASSOCIATION</publisher><subject>Animals ; Antibiotics, Antineoplastic - analysis ; Antineoplastic agents ; Antineoplastic Agents, Phytogenic - analysis ; Biological and medical sciences ; Biological Assay ; Cell Line ; Cell Survival - drug effects ; Colonic Neoplasms - pathology ; DNA - metabolism ; Dose-Response Relationship, Drug ; Drug Evaluation, Preclinical - methods ; Fermentation ; General aspects ; Humans ; Medical sciences ; Melanoma - pathology ; Mice ; Pharmacology. Drug treatments</subject><ispartof>The Journal of Antibiotics, 1985, Vol.38(6), pp.758-766</ispartof><rights>Japan Antibiotics Research Association</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5218-62066eed18bc228ab50dcfccab36eb6b4e3cb3f0e76249760c7cfde7ebd9a3753</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8418426$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4019319$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MIRABELLI, CHRISTOPHER K.</creatorcontrib><creatorcontrib>BARTUS, HENRY</creatorcontrib><creatorcontrib>BARTUS, JOAN O. L.</creatorcontrib><creatorcontrib>JOHNSON, RANDALL</creatorcontrib><creatorcontrib>MONG, SHAU MING</creatorcontrib><creatorcontrib>SUNG, CHENG PO</creatorcontrib><creatorcontrib>CROOKE, STANLEY T.</creatorcontrib><title>APPLICATION OF A TISSUE CULTURE MICROTITER TEST FOR THE DETECTION OF CYTOTOXIC AGENTS FROM NATURAL PRODUCTS</title><title>Journal of antibiotics</title><addtitle>J. Antibiot.</addtitle><description>A method is described by which the growth inhibitory effects of cytotoxic compounds and fermentation broth cultures on adherent tumor cell lines can be quantitated. Cells are seeded into 96-well microtiter plates and 16 hours later the test compounds or broths are added to the wells. Cell growth is measured after three days (B16 mouse melanoma cells) or six days (HT-29, human colon carcinoma cells) by first fixing adherent cells, staining with Giemsa stain, washing away excess stain, then solubilizing stained cells with HCl. Absorbance is determined using a microELISA spectrophotometer and the data are transferred to and analyzed by a computer. The assay is rapid and reproducible and can be used to identify fermentation broths with cytotoxic components. Addition of DNA into the assay mixture (cells plus compound) inhibits the cytotoxic activities of certain DNA-reactive agents. The results of this study demonstrate the application of this assay system for primary and secondary evaluation of fermentation broths for in vitro antitumor activity.</description><subject>Animals</subject><subject>Antibiotics, Antineoplastic - analysis</subject><subject>Antineoplastic agents</subject><subject>Antineoplastic Agents, Phytogenic - analysis</subject><subject>Biological and medical sciences</subject><subject>Biological Assay</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Colonic Neoplasms - pathology</subject><subject>DNA - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Evaluation, Preclinical - methods</subject><subject>Fermentation</subject><subject>General aspects</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Melanoma - pathology</subject><subject>Mice</subject><subject>Pharmacology. Drug treatments</subject><issn>0021-8820</issn><issn>1881-1469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNpNkM2O0zAUhS0EGsrACyAheYHYpfgnsZ1llHFnIjJNlTgSrCzHcSBD2g5xu-DtcdVSzcbX0vnOPVcHgI8YLTlm8VezO4zduD-M1i-pWPJEvAILLASOcMzS12CBEMGREAS9Be-8f0KIcsrFDbiJEU4pThfgd7bZlEWeqaJaw2oFM6iKpmklzNtStbWEj0VeV6pQsoZKNgquqvB5kPBOKpn_d-U_VKWq70UOs3u5Vg1c1dUjXGdhQ1bCTV3dtblq3oM3g5m8-3CZt6BdSZU_RGV1H04oI5sQLCJGEGPO9Vh0lhBhugT1drDWdJS5jnWxo7ajA3KckTjlDFluh95x1_WpoTyht-DLee_zvP9zdP6gt6O3bprMzu2PXgcfIQk9geQM2nnv_ewG_TyPWzP_1RjpU8P6RcOaCh0aDqZPl-3Hbuv6q-VSadA_X3TjrZmG2ezs6K-YiLGICQvYtzP25A_mp7vqZg5hk3uZjFMmTuns_IQjrpT9ZWbtdvQf0CWagw</recordid><startdate>1985</startdate><enddate>1985</enddate><creator>MIRABELLI, CHRISTOPHER K.</creator><creator>BARTUS, HENRY</creator><creator>BARTUS, JOAN O. 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L. ; JOHNSON, RANDALL ; MONG, SHAU MING ; SUNG, CHENG PO ; CROOKE, STANLEY T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5218-62066eed18bc228ab50dcfccab36eb6b4e3cb3f0e76249760c7cfde7ebd9a3753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Antibiotics, Antineoplastic - analysis</topic><topic>Antineoplastic agents</topic><topic>Antineoplastic Agents, Phytogenic - analysis</topic><topic>Biological and medical sciences</topic><topic>Biological Assay</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Colonic Neoplasms - pathology</topic><topic>DNA - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Evaluation, Preclinical - methods</topic><topic>Fermentation</topic><topic>General aspects</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Melanoma - pathology</topic><topic>Mice</topic><topic>Pharmacology. 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| fulltext | fulltext |
| identifier | ISSN: 0021-8820 |
| ispartof | The Journal of Antibiotics, 1985, Vol.38(6), pp.758-766 |
| issn | 0021-8820 1881-1469 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76222535 |
| source | J-STAGE (Japan Science & Technology Information Aggregator, Electronic) - Open Access English articles |
| subjects | Animals Antibiotics, Antineoplastic - analysis Antineoplastic agents Antineoplastic Agents, Phytogenic - analysis Biological and medical sciences Biological Assay Cell Line Cell Survival - drug effects Colonic Neoplasms - pathology DNA - metabolism Dose-Response Relationship, Drug Drug Evaluation, Preclinical - methods Fermentation General aspects Humans Medical sciences Melanoma - pathology Mice Pharmacology. Drug treatments |
| title | APPLICATION OF A TISSUE CULTURE MICROTITER TEST FOR THE DETECTION OF CYTOTOXIC AGENTS FROM NATURAL PRODUCTS |
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