Identification of upregulated immune-related genes in Vibrio harveyi challenged Penaeus monodon postlarvae

A subtracted cDNA library was constructed and analyzed to elucidate the response of Penaeus monodon postlarvae challenged with Vibrio harveyi. As many as 960 randomly selected cDNA fragments generated through suppression subtractive hybridization were single pass sequenced. Forty five genes and 20 h...

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Bibliographic Details
Published in:Fish & shellfish immunology 2010-09, Vol.29 (3), p.544-549
Main Authors: Nayak, S., Singh, S.K., Ramaiah, N., Sreepada, R.A.
Format: Article
Language:English
Subjects:
Animals
EST
Gene Expression Profiling
Immunity, Innate - genetics
Larva
Penaeidae - genetics
Penaeidae - immunology
Penaeidae - microbiology
Penaeus monodon
qPCR
Shrimp diseases
SSH
Up-Regulation
Vibrio - immunology
Vibrio harveyi
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Summary:A subtracted cDNA library was constructed and analyzed to elucidate the response of Penaeus monodon postlarvae challenged with Vibrio harveyi. As many as 960 randomly selected cDNA fragments generated through suppression subtractive hybridization were single pass sequenced. Forty five genes and 20 hypothetical proteins were identified, a few being first reports from shrimps. The most abundant immune relevant genes were ferritin, hemocyanin, and TCTP (translationally controlled tumor protein) indicating their upregulation as also confirmed through qPCR. Post-infection qPCR analyses confirmed 2.04, 2.09, 3.28, 5.49, 6.47, and 11.63 fold rise respectively in ferritin, penaeidin, MnSOD, lysozyme, TCTP, and hemocyanin genes. These genes may be involved in the regulation of the host defense against V. harveyi.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2010.05.010