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Trypanosoma cruzi: Modulation of HSP70 mRNA stability by untranslated regions during heat shock
Gene regulation in trypanosomatids occurs mainly by post-transcriptional mechanisms modulating mRNA stability and translation. We have investigated heat shock protein (HSP) 70 gene regulation in Trypanosoma cruzi, the causal agent of Chagas’ disease. The HSP70 mRNA’s half-life increases after heat s...
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| Published in: | Experimental parasitology 2010-10, Vol.126 (2), p.245-253 |
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| container_title | Experimental parasitology |
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| creator | Rodrigues, Deivid C Silva, Rosane Rondinelli, Edson Ürményi, Turán P |
| description | Gene regulation in trypanosomatids occurs mainly by post-transcriptional mechanisms modulating mRNA stability and translation. We have investigated heat shock protein (HSP) 70 gene regulation in
Trypanosoma cruzi, the causal agent of Chagas’ disease. The
HSP70 mRNA’s half-life increases after heat shock, and the stabilization is dependent on protein synthesis. In a cell-free RNA decay assay, a U-rich region in the 3′ untranslated region (UTR) is a target for degradation, which is reduced when in the presence of protein extracts from heat shocked cells. In a transfected reporter gene assay, both the 5′- and 3′-UTRs confer temperature-dependent regulation. Both UTRs must be present to increase mRNA stability at 37
°C, indicating that the 5′- and 3′-UTRs act cooperatively to stabilize
HSP70 mRNA during heat shock. We conclude that
HSP70 5′- and 3′-UTRs regulate mRNA stability during heat shock in
T. cruzi. |
| doi_str_mv | 10.1016/j.exppara.2010.05.009 |
| format | article |
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Trypanosoma cruzi, the causal agent of Chagas’ disease. The
HSP70 mRNA’s half-life increases after heat shock, and the stabilization is dependent on protein synthesis. In a cell-free RNA decay assay, a U-rich region in the 3′ untranslated region (UTR) is a target for degradation, which is reduced when in the presence of protein extracts from heat shocked cells. In a transfected reporter gene assay, both the 5′- and 3′-UTRs confer temperature-dependent regulation. Both UTRs must be present to increase mRNA stability at 37
°C, indicating that the 5′- and 3′-UTRs act cooperatively to stabilize
HSP70 mRNA during heat shock. We conclude that
HSP70 5′- and 3′-UTRs regulate mRNA stability during heat shock in
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Trypanosoma cruzi, the causal agent of Chagas’ disease. The
HSP70 mRNA’s half-life increases after heat shock, and the stabilization is dependent on protein synthesis. In a cell-free RNA decay assay, a U-rich region in the 3′ untranslated region (UTR) is a target for degradation, which is reduced when in the presence of protein extracts from heat shocked cells. In a transfected reporter gene assay, both the 5′- and 3′-UTRs confer temperature-dependent regulation. Both UTRs must be present to increase mRNA stability at 37
°C, indicating that the 5′- and 3′-UTRs act cooperatively to stabilize
HSP70 mRNA during heat shock. We conclude that
HSP70 5′- and 3′-UTRs regulate mRNA stability during heat shock in
T. cruzi.</description><subject>3' Untranslated Regions - genetics</subject><subject>3' Untranslated Regions - physiology</subject><subject>5' Untranslated Regions - genetics</subject><subject>5' Untranslated Regions - physiology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Chloramphenicol O-Acetyltransferase - genetics</subject><subject>Chloramphenicol O-Acetyltransferase - metabolism</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>DNA, Protozoan - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - physiology</subject><subject>Gene regulation</subject><subject>Half-Life</subject><subject>Hot Temperature</subject><subject>HSP70</subject><subject>HSP70 Heat-Shock Proteins - genetics</subject><subject>HSP70 Heat-Shock Proteins - metabolism</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>mRNA stability</subject><subject>Plasmids - genetics</subject><subject>Plasmids - metabolism</subject><subject>Protozoa</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Transfection</subject><subject>Trypanosoma cruzi</subject><subject>Trypanosoma cruzi - genetics</subject><subject>Trypanosoma cruzi - metabolism</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkUFv1DAQhS1ERbeFnwDyBXHKMnbsJOaCqqqlSC0gKGfLdiatlyQOdoK6_Hq82gWOPY1m9M3M03uEvGSwZsCqt5s1PkyTiWbNIc9ArgHUE7JioKDgQqinZAXARCEaJY7JSUobAGgYF8_IMQehykbIFdG3cTuZMaQwGOri8tu_ozehXXoz-zDS0NGrb19qoMPXT2c0zcb63s9bard0GedoxpRBbGnEu4wn2i7Rj3f0Hs1M031wP56To870CV8c6in5fnlxe35VXH_-8PH87Lpwoi7nojG2EZ1kHDhXqmmtKaGuuASrOmcdiKZigLbadZLb0rrOYGmZqZsOW8vKU_Jmf3eK4eeCadaDTw773owYlqTzMV5VnInHyWxYKWslMyn3pIshpYidnqIfTNxqBnoXgt7oQwh6F4IGqXMIee_V4cNiB2z_bf11PQOvD4BJzvRd9tH59J8rmzoL5Zl7v-cwO_fLY9TJeRwdtj6im3Ub_CNS_gDY7qhu</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Rodrigues, Deivid C</creator><creator>Silva, Rosane</creator><creator>Rondinelli, Edson</creator><creator>Ürményi, Turán P</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>20101001</creationdate><title>Trypanosoma cruzi: Modulation of HSP70 mRNA stability by untranslated regions during heat shock</title><author>Rodrigues, Deivid C ; Silva, Rosane ; Rondinelli, Edson ; Ürményi, Turán P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-8ab84f512022998dba3076250b9fcbc048610eb69fcb52b3bcfae3b1a78fedb13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>3' Untranslated Regions - genetics</topic><topic>3' Untranslated Regions - physiology</topic><topic>5' Untranslated Regions - genetics</topic><topic>5' Untranslated Regions - physiology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Chloramphenicol O-Acetyltransferase - genetics</topic><topic>Chloramphenicol O-Acetyltransferase - metabolism</topic><topic>DNA, Protozoan - chemistry</topic><topic>DNA, Protozoan - genetics</topic><topic>DNA, Protozoan - metabolism</topic><topic>Fundamental and applied biological sciences. 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We have investigated heat shock protein (HSP) 70 gene regulation in
Trypanosoma cruzi, the causal agent of Chagas’ disease. The
HSP70 mRNA’s half-life increases after heat shock, and the stabilization is dependent on protein synthesis. In a cell-free RNA decay assay, a U-rich region in the 3′ untranslated region (UTR) is a target for degradation, which is reduced when in the presence of protein extracts from heat shocked cells. In a transfected reporter gene assay, both the 5′- and 3′-UTRs confer temperature-dependent regulation. Both UTRs must be present to increase mRNA stability at 37
°C, indicating that the 5′- and 3′-UTRs act cooperatively to stabilize
HSP70 mRNA during heat shock. We conclude that
HSP70 5′- and 3′-UTRs regulate mRNA stability during heat shock in
T. cruzi.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>20493845</pmid><doi>10.1016/j.exppara.2010.05.009</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Experimental parasitology, 2010-10, Vol.126 (2), p.245-253 |
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| language | eng |
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| source | ScienceDirect Journals |
| subjects | 3' Untranslated Regions - genetics 3' Untranslated Regions - physiology 5' Untranslated Regions - genetics 5' Untranslated Regions - physiology Base Sequence Biological and medical sciences Blotting, Northern Chloramphenicol O-Acetyltransferase - genetics Chloramphenicol O-Acetyltransferase - metabolism DNA, Protozoan - chemistry DNA, Protozoan - genetics DNA, Protozoan - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation - physiology Gene regulation Half-Life Hot Temperature HSP70 HSP70 Heat-Shock Proteins - genetics HSP70 Heat-Shock Proteins - metabolism Life cycle. Host-agent relationship. Pathogenesis mRNA stability Plasmids - genetics Plasmids - metabolism Protozoa Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism Transfection Trypanosoma cruzi Trypanosoma cruzi - genetics Trypanosoma cruzi - metabolism |
| title | Trypanosoma cruzi: Modulation of HSP70 mRNA stability by untranslated regions during heat shock |
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