Role of differential expression of sdh1-1 and sdh1-2 genes in alteration of isoenzyme composition of succinate dehydrogenase in germinating maize seeds

A probable mechanism of alteration of the isoenzyme composition of succinate dehydrogenase (SDH) due to differential expression of genes encoding subunit A was considered. The alteration of SDH activity during maize seed germination was investigated, and its maximal activity on day 4-5 of germinatio...

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Bibliographic Details
Published in:Biology bulletin of the Russian Academy of Sciences 2010-06, Vol.37 (3), p.268-276
Main Authors: Eprintsev, A. T, Fedorin, D. N, Selivanova, N. V, Akhmad, G. A, Popov, V. N
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Cell Biology
Ecology
Isoenzymes
Life Sciences
Plant Physiology
Seeds
Succinate dehydrogenase
Zea mays
Zoology
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Summary:A probable mechanism of alteration of the isoenzyme composition of succinate dehydrogenase (SDH) due to differential expression of genes encoding subunit A was considered. The alteration of SDH activity during maize seed germination was investigated, and its maximal activity on day 4-5 of germination was found. The alteration of the sdh1-1 and sdh1-2 gene expression level during maize seed germination was evaluated using the quantitative polymerase chain reaction method. The presence of four forms of the studied enzymes, providing multiple SDH functions was found in maize inflorescence using electrophoresis in polyacrylamide gel.
ISSN:1062-3590
1608-3059
DOI:10.1134/S1062359010030088