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Studies on bitter gourd peroxidase catalyzed removal of p-bromophenol from wastewater
In the present study a systematic treatment of p-bromophenol has been optimized using a peroxidase derived from bitter gourd. Maximum 94% removal of the targeted phenol was obtained in presence of 0.4 U mL − 1 of peroxidase, 0.75 mM H 2O 2, and 0.1 mg mL − 1 of an additive, polyethylene glycol. Howe...
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| Published in: | Desalination 2010-11, Vol.262 (1), p.267-272 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c398t-b8503bc142d6acbd2ded3e0360de756f8d02850b5092877e21df285a86f4276a3 |
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| cites | cdi_FETCH-LOGICAL-c398t-b8503bc142d6acbd2ded3e0360de756f8d02850b5092877e21df285a86f4276a3 |
| container_end_page | 272 |
| container_issue | 1 |
| container_start_page | 267 |
| container_title | Desalination |
| container_volume | 262 |
| creator | Ashraf, Humaira Husain, Qayyum |
| description | In the present study a systematic treatment of
p-bromophenol has been optimized using a peroxidase derived from bitter gourd. Maximum 94% removal of the targeted phenol was obtained in presence of 0.4
U
mL
−
1 of peroxidase, 0.75
mM H
2O
2, and 0.1
mg
mL
−
1 of an additive, polyethylene glycol. However, in absence of polyethylene glycol about 1.2
U
mL
−
1 of peroxidase was able to remove about 70% of the phenolic compound. This enzyme showed about 32% of
p-bromophenol removal in the presence of 0.1
mM sodium azide and polyethylene glycol while as the enzyme was completely inhibited by sodium azide in absence of an additive. Hence, the present results demonstrate a good potentiality of bitter gourd peroxidase towards the treatment of halogenated phenolic compound in presence of an additive. Treated compound exhibited low toxicity as demonstrated by
Allium cepa test. UV absorbance spectra showed that there was a remarkable decrease in absorption peak for the treated compound. |
| doi_str_mv | 10.1016/j.desal.2010.05.044 |
| format | article |
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p-bromophenol has been optimized using a peroxidase derived from bitter gourd. Maximum 94% removal of the targeted phenol was obtained in presence of 0.4
U
mL
−
1 of peroxidase, 0.75
mM H
2O
2, and 0.1
mg
mL
−
1 of an additive, polyethylene glycol. However, in absence of polyethylene glycol about 1.2
U
mL
−
1 of peroxidase was able to remove about 70% of the phenolic compound. This enzyme showed about 32% of
p-bromophenol removal in the presence of 0.1
mM sodium azide and polyethylene glycol while as the enzyme was completely inhibited by sodium azide in absence of an additive. Hence, the present results demonstrate a good potentiality of bitter gourd peroxidase towards the treatment of halogenated phenolic compound in presence of an additive. Treated compound exhibited low toxicity as demonstrated by
Allium cepa test. UV absorbance spectra showed that there was a remarkable decrease in absorption peak for the treated compound.</description><identifier>ISSN: 0011-9164</identifier><identifier>EISSN: 1873-4464</identifier><identifier>DOI: 10.1016/j.desal.2010.05.044</identifier><identifier>CODEN: DSLNAH</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Additives ; Allium cepa ; Applied sciences ; Batch process ; Catalysis ; Catalytic reactions ; Chemical engineering ; Chemistry ; Enzymes ; Exact sciences and technology ; General and physical chemistry ; General purification processes ; Gourds ; Momordica charantia ; Peroxidase ; Pollution ; Polyethylene glycol ; Reactors ; Sodium azides ; Spectra ; Theory of reactions, general kinetics. Catalysis. Nomenclature, chemical documentation, computer chemistry ; Toxicity ; Waste water ; Wastewater ; Wastewaters ; Water treatment and pollution</subject><ispartof>Desalination, 2010-11, Vol.262 (1), p.267-272</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-b8503bc142d6acbd2ded3e0360de756f8d02850b5092877e21df285a86f4276a3</citedby><cites>FETCH-LOGICAL-c398t-b8503bc142d6acbd2ded3e0360de756f8d02850b5092877e21df285a86f4276a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23270098$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Ashraf, Humaira</creatorcontrib><creatorcontrib>Husain, Qayyum</creatorcontrib><title>Studies on bitter gourd peroxidase catalyzed removal of p-bromophenol from wastewater</title><title>Desalination</title><description>In the present study a systematic treatment of
p-bromophenol has been optimized using a peroxidase derived from bitter gourd. Maximum 94% removal of the targeted phenol was obtained in presence of 0.4
U
mL
−
1 of peroxidase, 0.75
mM H
2O
2, and 0.1
mg
mL
−
1 of an additive, polyethylene glycol. However, in absence of polyethylene glycol about 1.2
U
mL
−
1 of peroxidase was able to remove about 70% of the phenolic compound. This enzyme showed about 32% of
p-bromophenol removal in the presence of 0.1
mM sodium azide and polyethylene glycol while as the enzyme was completely inhibited by sodium azide in absence of an additive. Hence, the present results demonstrate a good potentiality of bitter gourd peroxidase towards the treatment of halogenated phenolic compound in presence of an additive. Treated compound exhibited low toxicity as demonstrated by
Allium cepa test. UV absorbance spectra showed that there was a remarkable decrease in absorption peak for the treated compound.</description><subject>Additives</subject><subject>Allium cepa</subject><subject>Applied sciences</subject><subject>Batch process</subject><subject>Catalysis</subject><subject>Catalytic reactions</subject><subject>Chemical engineering</subject><subject>Chemistry</subject><subject>Enzymes</subject><subject>Exact sciences and technology</subject><subject>General and physical chemistry</subject><subject>General purification processes</subject><subject>Gourds</subject><subject>Momordica charantia</subject><subject>Peroxidase</subject><subject>Pollution</subject><subject>Polyethylene glycol</subject><subject>Reactors</subject><subject>Sodium azides</subject><subject>Spectra</subject><subject>Theory of reactions, general kinetics. Catalysis. 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Catalysis. Nomenclature, chemical documentation, computer chemistry</topic><topic>Toxicity</topic><topic>Waste water</topic><topic>Wastewater</topic><topic>Wastewaters</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ashraf, Humaira</creatorcontrib><creatorcontrib>Husain, Qayyum</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Environmental Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><collection>Aqualine</collection><collection>Environment Abstracts</collection><collection>Water Resources Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Environment Abstracts</collection><jtitle>Desalination</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ashraf, Humaira</au><au>Husain, Qayyum</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on bitter gourd peroxidase catalyzed removal of p-bromophenol from wastewater</atitle><jtitle>Desalination</jtitle><date>2010-11-15</date><risdate>2010</risdate><volume>262</volume><issue>1</issue><spage>267</spage><epage>272</epage><pages>267-272</pages><issn>0011-9164</issn><eissn>1873-4464</eissn><coden>DSLNAH</coden><abstract>In the present study a systematic treatment of
p-bromophenol has been optimized using a peroxidase derived from bitter gourd. Maximum 94% removal of the targeted phenol was obtained in presence of 0.4
U
mL
−
1 of peroxidase, 0.75
mM H
2O
2, and 0.1
mg
mL
−
1 of an additive, polyethylene glycol. However, in absence of polyethylene glycol about 1.2
U
mL
−
1 of peroxidase was able to remove about 70% of the phenolic compound. This enzyme showed about 32% of
p-bromophenol removal in the presence of 0.1
mM sodium azide and polyethylene glycol while as the enzyme was completely inhibited by sodium azide in absence of an additive. Hence, the present results demonstrate a good potentiality of bitter gourd peroxidase towards the treatment of halogenated phenolic compound in presence of an additive. Treated compound exhibited low toxicity as demonstrated by
Allium cepa test. UV absorbance spectra showed that there was a remarkable decrease in absorption peak for the treated compound.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.desal.2010.05.044</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0011-9164 |
| ispartof | Desalination, 2010-11, Vol.262 (1), p.267-272 |
| issn | 0011-9164 1873-4464 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_762266887 |
| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Additives Allium cepa Applied sciences Batch process Catalysis Catalytic reactions Chemical engineering Chemistry Enzymes Exact sciences and technology General and physical chemistry General purification processes Gourds Momordica charantia Peroxidase Pollution Polyethylene glycol Reactors Sodium azides Spectra Theory of reactions, general kinetics. Catalysis. Nomenclature, chemical documentation, computer chemistry Toxicity Waste water Wastewater Wastewaters Water treatment and pollution |
| title | Studies on bitter gourd peroxidase catalyzed removal of p-bromophenol from wastewater |
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