Loading…

Dietary DNA in blood and organs of Atlantic salmon (Salmo salar L.)

The objective of this study was to investigate the uptake of dietary DNA into blood, kidney, and liver of salmon, and to determine the DNA fragment size if dietary DNA was detected. Salmon in groups of five fish were force-fed a feed containing a high copy number of three polymerase chain reaction (...

Full description

Saved in:
Bibliographic Details
Published in:European food research & technology 2005-07, Vol.221 (1-2), p.1-8
Main Authors: Nielsen, C.R, Berdal, K.G, Bakke-McKellep, A.M, Holst-Jensen, A
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c358t-98632699a33920a812c6807a0e55f5f18742372d9f4d209f1efb4271e99dafeb3
cites cdi_FETCH-LOGICAL-c358t-98632699a33920a812c6807a0e55f5f18742372d9f4d209f1efb4271e99dafeb3
container_end_page 8
container_issue 1-2
container_start_page 1
container_title European food research & technology
container_volume 221
creator Nielsen, C.R
Berdal, K.G
Bakke-McKellep, A.M
Holst-Jensen, A
description The objective of this study was to investigate the uptake of dietary DNA into blood, kidney, and liver of salmon, and to determine the DNA fragment size if dietary DNA was detected. Salmon in groups of five fish were force-fed a feed containing a high copy number of three polymerase chain reaction (PCR) amplified DNA fragments. Tissue samples were dissected from the fish at time intervals starting at 1 h after force-feeding (AFF) and ending at 64 h AFF. Real-time PCR analyses were used to determine the presence or absence of DNA targets. Sensitive methods amplifying small fragments were used to minimise the impact of fragmentation on the detectability of DNA targets. Uptake of dietary DNA was observed and the highest concentrations of dietary DNA in liver and kidney were found 8 h AFF. The results correspond to data published for similar trials performed on other animal species. An additional experiment showed that decontamination of the liver surface by flaming has the potential to decrease DNA contamination from, for example, feed remnants by up to 90%.[PUBLICATION ABSTRACT]
doi_str_mv 10.1007/s00217-005-1160-1
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_762270498</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>762270498</sourcerecordid><originalsourceid>FETCH-LOGICAL-c358t-98632699a33920a812c6807a0e55f5f18742372d9f4d209f1efb4271e99dafeb3</originalsourceid><addsrcrecordid>eNpdkE1LAzEQhhdRsFZ_gCeDIOph60yym49jaf2Coofac0h3k7Jlu9Fke_Dfm9Ki4Glm4HmHlyfLLhFGCCAeIgBFkQOUOSKHHI-yARZM5pTJ8vh3F-I0O4txnTjFsRhkk2ljexO-yfRtTJqOLFvva2K6mviwMl0k3pFx35qubyoSTbvxHbmb7-buMoHMRvfn2YkzbbQXhznMFk-PH5OXfPb-_DoZz_KKlbLPleSMcqUMY4qCkUgrLkEYsGXpSodSFKkgrZUragrKoXXLggq0StXG2SUbZrf7v5_Bf21t7PWmiZVtUzvrt1ELTqmAQslEXv8j134bulROpw5QUsFZgnAPVcHHGKzTn6HZJBcaQe-k6r1UnWTpnVSNKXNzeGxiZVoXTFc18S_IVakKAYm72nPOeG1WITGLOQVkgMCZ4JL9ALgEfA0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>632052763</pqid></control><display><type>article</type><title>Dietary DNA in blood and organs of Atlantic salmon (Salmo salar L.)</title><source>ABI/INFORM Global</source><source>Springer Link</source><creator>Nielsen, C.R ; Berdal, K.G ; Bakke-McKellep, A.M ; Holst-Jensen, A</creator><creatorcontrib>Nielsen, C.R ; Berdal, K.G ; Bakke-McKellep, A.M ; Holst-Jensen, A</creatorcontrib><description>The objective of this study was to investigate the uptake of dietary DNA into blood, kidney, and liver of salmon, and to determine the DNA fragment size if dietary DNA was detected. Salmon in groups of five fish were force-fed a feed containing a high copy number of three polymerase chain reaction (PCR) amplified DNA fragments. Tissue samples were dissected from the fish at time intervals starting at 1 h after force-feeding (AFF) and ending at 64 h AFF. Real-time PCR analyses were used to determine the presence or absence of DNA targets. Sensitive methods amplifying small fragments were used to minimise the impact of fragmentation on the detectability of DNA targets. Uptake of dietary DNA was observed and the highest concentrations of dietary DNA in liver and kidney were found 8 h AFF. The results correspond to data published for similar trials performed on other animal species. An additional experiment showed that decontamination of the liver surface by flaming has the potential to decrease DNA contamination from, for example, feed remnants by up to 90%.[PUBLICATION ABSTRACT]</description><identifier>ISSN: 1438-2377</identifier><identifier>EISSN: 1438-2385</identifier><identifier>DOI: 10.1007/s00217-005-1160-1</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>animal organs ; Animal species ; Biological and medical sciences ; Blood ; Decontamination ; Deoxyribonucleic acid ; detection ; DNA ; fish ; Fish and seafood industries ; food composition ; Food industries ; Fundamental and applied biological sciences. Psychology ; genetically modified organisms ; intestinal absorption ; Kidneys ; liver ; Marine ; molecular sequence data ; nucleotide sequences ; polymerase chain reaction ; reporter genes ; Salmo salar ; Salmon ; temporal variation ; transgenes</subject><ispartof>European food research &amp; technology, 2005-07, Vol.221 (1-2), p.1-8</ispartof><rights>2005 INIST-CNRS</rights><rights>Springer-Verlag 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-98632699a33920a812c6807a0e55f5f18742372d9f4d209f1efb4271e99dafeb3</citedby><cites>FETCH-LOGICAL-c358t-98632699a33920a812c6807a0e55f5f18742372d9f4d209f1efb4271e99dafeb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/632052763/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/632052763?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,11688,27924,27925,36060,36061,44363,74895</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=16959470$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Nielsen, C.R</creatorcontrib><creatorcontrib>Berdal, K.G</creatorcontrib><creatorcontrib>Bakke-McKellep, A.M</creatorcontrib><creatorcontrib>Holst-Jensen, A</creatorcontrib><title>Dietary DNA in blood and organs of Atlantic salmon (Salmo salar L.)</title><title>European food research &amp; technology</title><description>The objective of this study was to investigate the uptake of dietary DNA into blood, kidney, and liver of salmon, and to determine the DNA fragment size if dietary DNA was detected. Salmon in groups of five fish were force-fed a feed containing a high copy number of three polymerase chain reaction (PCR) amplified DNA fragments. Tissue samples were dissected from the fish at time intervals starting at 1 h after force-feeding (AFF) and ending at 64 h AFF. Real-time PCR analyses were used to determine the presence or absence of DNA targets. Sensitive methods amplifying small fragments were used to minimise the impact of fragmentation on the detectability of DNA targets. Uptake of dietary DNA was observed and the highest concentrations of dietary DNA in liver and kidney were found 8 h AFF. The results correspond to data published for similar trials performed on other animal species. An additional experiment showed that decontamination of the liver surface by flaming has the potential to decrease DNA contamination from, for example, feed remnants by up to 90%.[PUBLICATION ABSTRACT]</description><subject>animal organs</subject><subject>Animal species</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Decontamination</subject><subject>Deoxyribonucleic acid</subject><subject>detection</subject><subject>DNA</subject><subject>fish</subject><subject>Fish and seafood industries</subject><subject>food composition</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genetically modified organisms</subject><subject>intestinal absorption</subject><subject>Kidneys</subject><subject>liver</subject><subject>Marine</subject><subject>molecular sequence data</subject><subject>nucleotide sequences</subject><subject>polymerase chain reaction</subject><subject>reporter genes</subject><subject>Salmo salar</subject><subject>Salmon</subject><subject>temporal variation</subject><subject>transgenes</subject><issn>1438-2377</issn><issn>1438-2385</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>M0C</sourceid><recordid>eNpdkE1LAzEQhhdRsFZ_gCeDIOph60yym49jaf2Coofac0h3k7Jlu9Fke_Dfm9Ki4Glm4HmHlyfLLhFGCCAeIgBFkQOUOSKHHI-yARZM5pTJ8vh3F-I0O4txnTjFsRhkk2ljexO-yfRtTJqOLFvva2K6mviwMl0k3pFx35qubyoSTbvxHbmb7-buMoHMRvfn2YkzbbQXhznMFk-PH5OXfPb-_DoZz_KKlbLPleSMcqUMY4qCkUgrLkEYsGXpSodSFKkgrZUragrKoXXLggq0StXG2SUbZrf7v5_Bf21t7PWmiZVtUzvrt1ELTqmAQslEXv8j134bulROpw5QUsFZgnAPVcHHGKzTn6HZJBcaQe-k6r1UnWTpnVSNKXNzeGxiZVoXTFc18S_IVakKAYm72nPOeG1WITGLOQVkgMCZ4JL9ALgEfA0</recordid><startdate>20050701</startdate><enddate>20050701</enddate><creator>Nielsen, C.R</creator><creator>Berdal, K.G</creator><creator>Bakke-McKellep, A.M</creator><creator>Holst-Jensen, A</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7QR</scope><scope>7RQ</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X2</scope><scope>7XB</scope><scope>87Z</scope><scope>88I</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>8FL</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>F~G</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>L.-</scope><scope>L6V</scope><scope>M0C</scope><scope>M0K</scope><scope>M2P</scope><scope>M7S</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7TM</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20050701</creationdate><title>Dietary DNA in blood and organs of Atlantic salmon (Salmo salar L.)</title><author>Nielsen, C.R ; Berdal, K.G ; Bakke-McKellep, A.M ; Holst-Jensen, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-98632699a33920a812c6807a0e55f5f18742372d9f4d209f1efb4271e99dafeb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>animal organs</topic><topic>Animal species</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Decontamination</topic><topic>Deoxyribonucleic acid</topic><topic>detection</topic><topic>DNA</topic><topic>fish</topic><topic>Fish and seafood industries</topic><topic>food composition</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genetically modified organisms</topic><topic>intestinal absorption</topic><topic>Kidneys</topic><topic>liver</topic><topic>Marine</topic><topic>molecular sequence data</topic><topic>nucleotide sequences</topic><topic>polymerase chain reaction</topic><topic>reporter genes</topic><topic>Salmo salar</topic><topic>Salmon</topic><topic>temporal variation</topic><topic>transgenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nielsen, C.R</creatorcontrib><creatorcontrib>Berdal, K.G</creatorcontrib><creatorcontrib>Bakke-McKellep, A.M</creatorcontrib><creatorcontrib>Holst-Jensen, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>ProQuest Career and Technical Education</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection</collection><collection>Science Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Engineering Collection</collection><collection>ABI/INFORM Global</collection><collection>Agriculture Science Database</collection><collection>ProQuest Science Journals</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>Nucleic Acids Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><jtitle>European food research &amp; technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nielsen, C.R</au><au>Berdal, K.G</au><au>Bakke-McKellep, A.M</au><au>Holst-Jensen, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dietary DNA in blood and organs of Atlantic salmon (Salmo salar L.)</atitle><jtitle>European food research &amp; technology</jtitle><date>2005-07-01</date><risdate>2005</risdate><volume>221</volume><issue>1-2</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>1438-2377</issn><eissn>1438-2385</eissn><abstract>The objective of this study was to investigate the uptake of dietary DNA into blood, kidney, and liver of salmon, and to determine the DNA fragment size if dietary DNA was detected. Salmon in groups of five fish were force-fed a feed containing a high copy number of three polymerase chain reaction (PCR) amplified DNA fragments. Tissue samples were dissected from the fish at time intervals starting at 1 h after force-feeding (AFF) and ending at 64 h AFF. Real-time PCR analyses were used to determine the presence or absence of DNA targets. Sensitive methods amplifying small fragments were used to minimise the impact of fragmentation on the detectability of DNA targets. Uptake of dietary DNA was observed and the highest concentrations of dietary DNA in liver and kidney were found 8 h AFF. The results correspond to data published for similar trials performed on other animal species. An additional experiment showed that decontamination of the liver surface by flaming has the potential to decrease DNA contamination from, for example, feed remnants by up to 90%.[PUBLICATION ABSTRACT]</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s00217-005-1160-1</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1438-2377
ispartof European food research & technology, 2005-07, Vol.221 (1-2), p.1-8
issn 1438-2377
1438-2385
language eng
recordid cdi_proquest_miscellaneous_762270498
source ABI/INFORM Global; Springer Link
subjects animal organs
Animal species
Biological and medical sciences
Blood
Decontamination
Deoxyribonucleic acid
detection
DNA
fish
Fish and seafood industries
food composition
Food industries
Fundamental and applied biological sciences. Psychology
genetically modified organisms
intestinal absorption
Kidneys
liver
Marine
molecular sequence data
nucleotide sequences
polymerase chain reaction
reporter genes
Salmo salar
Salmon
temporal variation
transgenes
title Dietary DNA in blood and organs of Atlantic salmon (Salmo salar L.)
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T23%3A19%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dietary%20DNA%20in%20blood%20and%20organs%20of%20Atlantic%20salmon%20(Salmo%20salar%20L.)&rft.jtitle=European%20food%20research%20&%20technology&rft.au=Nielsen,%20C.R&rft.date=2005-07-01&rft.volume=221&rft.issue=1-2&rft.spage=1&rft.epage=8&rft.pages=1-8&rft.issn=1438-2377&rft.eissn=1438-2385&rft_id=info:doi/10.1007/s00217-005-1160-1&rft_dat=%3Cproquest_cross%3E762270498%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c358t-98632699a33920a812c6807a0e55f5f18742372d9f4d209f1efb4271e99dafeb3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=632052763&rft_id=info:pmid/&rfr_iscdi=true