Biochemical and Physiochemical Properties of Actomyosin and Myofibrils from Frozen Stored Flounder (Paralichthys patagonicus) Fillets

The biochemical and physicochemical properties of myofibrils and actomyosin (AM) from frozen stored fillets of flounder were investigated. Solubility of proteins increased after freezing and decreased during frozen storage. No significant (P < 0.05) changes were detected in both Mg2+ (Ca2+) and M...

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Published in:Journal of food biochemistry 2010-10, Vol.34 (5), p.983-997
Main Authors: PAREDI, ME, Pagano, M R, CRUPKIN, M
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Language:English
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Marine
Paralichthys patagonicus
Pleuronectiformes
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Pagano, M R
CRUPKIN, M
description The biochemical and physicochemical properties of myofibrils and actomyosin (AM) from frozen stored fillets of flounder were investigated. Solubility of proteins increased after freezing and decreased during frozen storage. No significant (P < 0.05) changes were detected in both Mg2+ (Ca2+) and Mg2+ Ethylene glycol tetraacetic acid (EGTA) Adenosinetriphosphatase (ATPase) activities of myofibrils due to freezing and frozen storage. Freezing produced a significant (P < 0.05) increase in Mg2+ Ca2+ and Mg2+(EGTA) and Mg2+ATPase activities of AM. The (Ca2+) ATPase activity remained unchanged. All the ATPases activities of AM decreased during frozen storage. The changes in the enzymatic activities of AM during storage were accompanied by a decrease in the reduced viscosity of the protein. No signals of proteolysis were detected in the profiles of SDS-Polyacrilamyde Gel Electrophoresis 10% gels of both myofibrils and AM. A significant decrease (P < 0.05) in the relative percentage of myosin and myosin/actin ratio was observed at the end of storage. These results suggest that denaturation-aggregation of myosin occurs at the end of storage in frozen stored fillets.PRACTICAL APPLICATIONS
doi_str_mv 10.1111/j.1745-4514.2010.00344.x
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Paralichthys patagonicus
Pleuronectiformes
title Biochemical and Physiochemical Properties of Actomyosin and Myofibrils from Frozen Stored Flounder (Paralichthys patagonicus) Fillets
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