Activation of GABA-B receptors induced by systemic amphetamine abolishes dopamine release in the rat lateral septum

• Published in: Journal of neurochemistry 2010-09, Vol.114 (6), p.1678-1686
• Main Authors: Sotomayor-Zárate, Ramón, Araya, Katherine Angélica, Pereira, Paulo, Blanco, Elías, Quiroz, Gabriel, Pozo, Sergio, Carreño, Paz, Andrés, María Estela, Forray, María Inés, Gysling, Katia
• Format: Article
• Language: English
• Subjects: Addictive behaviors; Adult and adolescent clinical studies; Amphetamine; Amphetamine - pharmacology; Amphetamines; Animals; Anxiety; Benzylamines - pharmacology; Biological and medical sciences; Brain; Central nervous system; Central Nervous System Stimulants - pharmacology; Central neurotransmission. Neuromudulation. Pathways and receptors; Dialysis; Dopamine; Dopamine - metabolism; Drug addiction; Extracellular levels; Extracellular Space - metabolism; Fundamental and applied biological sciences. Psychology; g-Aminobutyric acid; g-Aminobutyric acid B receptors; GABA; GABA-B receptor; GABA-B Receptor Agonists; GABA-B Receptor Antagonists; gamma-Aminobutyric Acid - metabolism; Glutamate Decarboxylase - genetics; Glutamate Decarboxylase - metabolism; lateral septum; Male; Medical sciences; Mental disorders; Microdialysis; Neurochemistry; Neurons - drug effects; Neurons - metabolism; Neurotransmission; Nucleus accumbens; Nucleus Accumbens - drug effects; Nucleus Accumbens - metabolism; Perfusion; Phosphinic Acids - pharmacology; Probes; Psychology. Psychoanalysis. Psychiatry; Psychopathology. Psychiatry; Rats; Rats, Sprague-Dawley; RNA, Messenger - metabolism; Rodents; Septum; Septum of Brain - drug effects; Septum of Brain - metabolism; Vertebrates: nervous system and sense organs
• ISSN: 0022-3042; 1471-4159
• DOI: 10.1111/j.1471-4159.2010.06877.x

J. Neurochem. (2010) 114, 1678-1686. The lateral septum is a brain nucleus involved in various mental disorders such as anxiety and drug addiction. In the present study, we investigated whether systemic amphetamine, known to release dopamine (DA) in nucleus accumbens, will also release DA in lateral septum. Our results show that systemic amphetamine administration (2 mg/kg i.p.) induced a significant increase in DA extracellular levels in nucleus accumbens but not in lateral septum. Interestingly, intralateral septum perfusion of amphetamine through the microdialysis probe induced a significant increase in DA extracellular levels. To test if GABAergic neurotransmission in lateral septum was responsible for inhibiting the release of DA when amphetamine was administered systemically, we perfused a GABA-B selective antagonist (CGP-52432) intra lateral septum. Systemic amphetamine administration induced a significant increase in lateral septum DA release when CGP-52432 was concomitantly superfused. Our results indicate that the systemic administration of amphetamine induces an increase in lateral septum GABA release and the consequent activation of GABA-B receptors counteracting the direct effect of amphetamine on lateral septum DA release.