Adverse effect of the chitinolytic enzyme PjCHI-1 in transgenic tomato on egg mass production and embryonic development of Meloidogyne incognita

A novel chitinase gene (PjCHI-1) isolated from Paecilomyces javanicus, a non-nematophagous fungus, and driven by a CaMV35S promoter, was delivered into CLN2468D, a heat-tolerant cultivar of tomato (Solanum lycopersicum). T₁ tomato plants exhibited high endochitinase activity and reduced numbers of e...

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Bibliographic Details
Published in:Plant pathology 2010-10, Vol.59 (5), p.922-930
Main Authors: Chan, Y.-L, Cai, D, Taylor, P.W.J, Chan, M.-T, Yeh, K.W
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Biotechnology
Chitin
Chitinase
Crops
Eggs
Embryogenesis
Endochitinase
Enzymes
Fundamental and applied biological sciences. Psychology
fungal chitinase
Genetic engineering
Genetic engineering applications
Genetic technics
Genetics and breeding of economic plants
Lycopersicon esculentum
Meloidogyne incognita
Methods. Procedures. Technologies
Nematoda
Paecilomyces
Phytopathology. Animal pests. Plant and forest protection
PjCHI-1
Plant breeding: fundamental aspects and methodology
Promoters
Protozoa. Invertebrates
reproductivity
root-knot nematodes
root‐knot nematode
Shells
Side effects
Solanum
Solanum lycopersicum
Transgenic animals and transgenic plants
Transgenic plants
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Summary:A novel chitinase gene (PjCHI-1) isolated from Paecilomyces javanicus, a non-nematophagous fungus, and driven by a CaMV35S promoter, was delivered into CLN2468D, a heat-tolerant cultivar of tomato (Solanum lycopersicum). T₁ tomato plants exhibited high endochitinase activity and reduced numbers of eggs and egg masses when infected with the root-knot nematode (RKN) Meloidogyne incognita. The eggs found in transgenic tomato had lower shell chitin contents than eggs collected from control plants. Egg masses from transgenic plants exhibited higher chitinase activity than those from control plants. Moreover, only 30% of eggs from transgenic plants were able to develop to the multi-cell/J1 stage, compared with more than 96% from control plants. The present study demonstrated that the expression of the PjCHI-1 chitinase gene can effectively reduce the production of egg masses and repress the embryonic development of M. incognita, presenting the possibility of a novel agro-biotechnological strategy for preventing crop damage by RKN.
ISSN:0032-0862
1365-3059
DOI:10.1111/j.1365-3059.2010.02314.x