Sequence heterogeneity of the ferripyoverdine uptake (fpvA), but not the ferric uptake regulator (fur), genes among strains of the fluorescent pseudomonads Pseudomonas aeruginosa, Pseudomonas aureofaciens, Pseudomonas fluorescens and Pseudomonas putida

• Published in: Biometals 1999-09, Vol.12 (3), p.265-275
• Main Authors: Thupvong, T, Wiideman, A, Dunn, D, Oreschak, K, Jankowicz, B, Doering, J, Castignetti, D
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial Outer Membrane Proteins - genetics; Bacterial Proteins - genetics; Base Sequence; Burkholderia; Deoxyribonucleic acid; DNA, Bacterial - analysis; Enzymes; Genes; Genes, Bacterial; Genetic Heterogeneity; Heterogeneity; Iron - metabolism; Metalloproteins - genetics; Microbiology; Microorganisms; Polymorphism, Restriction Fragment Length; Pseudomonas; Pseudomonas - genetics; Pseudomonas aeruginosa; Pseudomonas aeruginosa - genetics; Pseudomonas aureofaciens; Pseudomonas cepacia; Pseudomonas fluorescens; Pseudomonas fluorescens - genetics; Pseudomonas putida; Pseudomonas putida - genetics; Regulators; Repressor Proteins - genetics; Sequence Analysis, DNA; Uptakes; Zinc - metabolism
• ISSN: 0966-0844; 1572-8773
• DOI: 10.1023/A:1009270302536

Pseudomonas aeruginosa, Pseudomonas aureofaciens, Pseudomonas fluorescens and Pseudomonas putida are of importance to medicine, agriculture and biocycling. These microbes acquire ferric ion via the use of the siderophores pyochelin and the family known as the pyoverdines or pseudobactins. The ferric uptake regulator (fur) gene is responsible, at least in part, for the regulation of siderophore synthesis and uptake in P. aeruginosa. To determine whether the organisms contain single or multiple homologues of the siderophore-related genes fpvA (ferripyoverdine uptake) and fur, and whether these homologues displayed sequence heterogeneity, their chromosomal DNAs were probed with fur and fpvA sequences. As a representative of a non-fluorescent pseudomonad, the bacterium Burkholderia (Pseudomonas) cepacia was also examined. The pseudomonads all contained fpvA- and fur-like homologues, and heterogeneity was observed among the different species. The presence of two or more fpvA-like genes is indicated in all of the fluorescent pseudomonads surveyed. In contrast, B. cepacia DNA either did not hybridize to these probes, or did so only very weakly, suggesting that fur- and fpvA-like homologues are either absent or significantly different in B. cepacia compared to the fluorescent pseudomonads examined.