integrated microfluidic chip for non-immunological determination of urinary albumin

This study presents an integrated microfluidic chip for non-immunologically determining the concentrations of albumin in clinical urine samples. This microchip integrates membrane-type micromixers and a fan-shaped micropump capable of simultaneously and precisely delivering assay reagents to react w...

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Bibliographic Details
Published in:Biomedical microdevices 2010-10, Vol.12 (5), p.887-896
Main Authors: Lin, Chun-Che, Tseng, Chin-Chung, Huang, Chao-June, Wang, Jung-Hao, Lee, Gwo-Bin
Format: Article
Language:English
Subjects:
Albuminuria - urine
Biological and Medical Physics
Biomedical Engineering and Bioengineering
Biophysics
Calibration
Chemical reactions
Engineering
Engineering Fluid Dynamics
Humans
immunoassays
Microalbuminuria
Microfluidic Analytical Techniques
Microfluidics
Micropump
Nanotechnology
Nephelometry and Turbidimetry
Proteins
Semiconductors
Surface Properties
Systems integration
Urinalysis
Urinalysis - instrumentation
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Summary:This study presents an integrated microfluidic chip for non-immunologically determining the concentrations of albumin in clinical urine samples. This microchip integrates membrane-type micromixers and a fan-shaped micropump capable of simultaneously and precisely delivering assay reagents to react with 6 urine samples in one single operation. The experimental results show that the coefficient of variation in the pumping rate is 2.42%. More importantly, using this unique chip design, only 2 electromagnetic valves are required for the actuation of the micromixer and the micropump. The working range of the proposed microchip is 2-200 mg/L of albumin, which covers the range of interest for the determination of microalbuminuria. Moreover, statistical analysis show that the results obtained by the proposed microchip are in good agreement with the conventional detection method, based on immunological assays. This simple, inexpensive and microchip-based platform presents a promising alternative to conventional immunological assays for measurement of urinary albumin, and is well suited for clinical applications.
ISSN:1387-2176
1572-8781
DOI:10.1007/s10544-010-9443-0