DNA fingerprinting of Paecilomyces strains of potential use for the biological control of pests

Telomeric fingerprinting was found to be highly differentiating for Paecilomyces fumosoroseus and Paecilomyces lilacinus isolates in comparison to intron splice site PCR and is therefore a good method for quality control of future products based on these fungi. Although the telomeric restriction len...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2005-12, Vol.21 (8-9), p.1487-1492
Main Authors: Inglis, P.W, Sarmento, R.B.C, Gaviao, C.F.C, Valadares-Inglis, M.C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological control
biological control agents
Biotechnology
Chromosomes
Deoxyribonucleic acid
DNA
DNA fingerprinting
entomopathogenic fungi
Fundamental and applied biological sciences. Psychology
introns
Paecilomyces
Paecilomyces fumosoroseus
Paecilomyces lilacinus
Pest control
polymerase chain reaction
Quality control
RNA splicing
telomeres
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Summary:Telomeric fingerprinting was found to be highly differentiating for Paecilomyces fumosoroseus and Paecilomyces lilacinus isolates in comparison to intron splice site PCR and is therefore a good method for quality control of future products based on these fungi. Although the telomeric restriction length polymorphisms correctly divided the isolates into their appropriate species, further correlation with host range or geographical origin of the isolates was not found. In this respect, intron splice site PCR was more informative taxonomically. The chromosome numbers inferred from telomeric fingerprints were seven chromosomes for P. lilacinus and between six and nine chromosomes for P. fumosoroseus.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-005-7224-3