An EPR Method for Studying Annexin-Biomembrane Interaction

Annexin p34 spin label has been used as a probe for studying annexin-membrane Ca-mediated interaction. The EPR spectrum of the purified probe is able to monitor the binding of the protein to phospholipid vesicles and isolated mitochondrial membranes, with increasing calcium concentration, as reveale...

Full description

Saved in:
Bibliographic Details
Published in:Analytical biochemistry 1993-11, Vol.214 (2), p.557-565
Main Authors: Megli, F.M., Selvaggi, M., Delisi, A., Quagliariello, E.
Format: Article
Language:English
Subjects:
Animals
Annexins - metabolism
Biological and medical sciences
Calcium - physiology
Electron Spin Resonance Spectroscopy
Fundamental and applied biological sciences. Psychology
Interactions. Associations
Intermolecular phenomena
Intracellular Membranes - metabolism
Mathematics
Mitochondria, Liver - metabolism
Molecular biophysics
Molecular Probes
Rats
Rotation
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Annexin p34 spin label has been used as a probe for studying annexin-membrane Ca-mediated interaction. The EPR spectrum of the purified probe is able to monitor the binding of the protein to phospholipid vesicles and isolated mitochondrial membranes, with increasing calcium concentration, as revealed by the calculated rotational correlation time. A novel mathematical approach is proposed to provide a direct estimation of annexin percentage binding to membranes, based on the correlation time calculated from experimental EPR spectra.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1993.1538