Somatic cell mapping of conglutinin (CGN1) to cattle syntenic group U29 and fluorescence in situ localization to Chromosome 28

A 260-bp genomic PstI fragment, which encodes a portion of the carbohydrate recognition domain, was used along with hybrid somatic cells to map the conglutinin gene (CGN1) to domestic cow (Bos taurus) syntenic group U29. In turn, a cosmid containing the entire bovine CGN1 was used with fluorescence...

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Bibliographic Details
Published in:Mammalian genome 1993-12, Vol.4 (12), p.716-719
Main Authors: Gallagher, Jr, D S, Ryan, A M, Liou, L S, Sastry, K N, Womack, J E
Format: Article
Language:English
Subjects:
Animals
Blotting, Southern - veterinary
Cattle - genetics
Chromosome Mapping - veterinary
Collectins
Hybrid Cells
In Situ Hybridization, Fluorescence
Serum Globulins - genetics
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Summary:A 260-bp genomic PstI fragment, which encodes a portion of the carbohydrate recognition domain, was used along with hybrid somatic cells to map the conglutinin gene (CGN1) to domestic cow (Bos taurus) syntenic group U29. In turn, a cosmid containing the entire bovine CGN1 was used with fluorescence in situ hybridization to sublocalize this gene to cattle Chromosome (Chr) (BTA) 28 band 18. Since BTA 28 and several of the other small acrocentric autosomes of cattle are difficult to discriminate, we have also chromosomally sublocalized CGN1 to the p arm of the lone biarmed autosome of the gaur (Bos gaurus). The use of the gaur 2/28 Robertsonian as a marker chromosome and our assignment of CGN1 to BTA 28 should help resolve some of the nomenclatural questions involving this cattle chromosome.
ISSN:0938-8990
1432-1777
DOI:10.1007/BF00357795