Fluorometric Determination of Phenylephrine Hydrochloride by Liquid Chromatography in Human Plasma

A sensitive method is described for the rapid determination of phenylephrine hydrochloride in human plasma. Phenylephrine was analyzed using high-performance liquid chromatography (HPLC) with fluorecence detection at excitation and emission wavelengths of 270 and 305 nm, respectively. A retention ti...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1985-05, Vol.74 (5), p.562-564
Main Authors: Chien, Du-Shieng, Schoenwald, Ronald D.
Format: Article
Language:English
Subjects:
Analysis
Applied sciences
Biological and medical sciences
Chromatography, High Pressure Liquid
Drug Stability
Exact sciences and technology
General pharmacology
Humans
Hydrogen-Ion Concentration
Medical sciences
Ophthalmic Solutions - analysis
Other techniques and industries
Pharmacology. Drug treatments
Phenylephrine - administration & dosage
Phenylephrine - blood
Spectrometry, Fluorescence
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Summary:A sensitive method is described for the rapid determination of phenylephrine hydrochloride in human plasma. Phenylephrine was analyzed using high-performance liquid chromatography (HPLC) with fluorecence detection at excitation and emission wavelengths of 270 and 305 nm, respectively. A retention time of ~4.25min was observed on chromatograms. Drug was separated from interfering plasma components by the use of disposable solid-phase extraction columns. Linear standard curves were constructed from drug concentrations of 0.5–5.0 and 2.5–80 ng/mL, for which an average recovery of 76–78% was obtained. Phenylephrine could be quantitatively measured at levels as low as 0.5 ng/mL. An assay precision (CV) of 23.6 and 15.9% was obtained at the lower sensitivities of 0.5 and 1.0 ng/mL, respectively. An example of the method was given for analysis of human plasma levels of drug following topical application of two drops of 2.5% aqueous or 10% viscous ophthalmic solution to the eyes of two subjects.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600740515