The use of continuous density gradients for the assessment of islet and exocrine tissue densities and islet purification

The purification of large numbers of human pancreatic islets remains one of the limiting factors in islet transplantation. This paper describes and validates a method for accurately and reproducibly determining the density of islets and exocrine tissue in pancreatic digest on the basis of their isop...

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Bibliographic Details
Published in:Acta diabetologica 1993, Vol.30 (3), p.175-180
Main Authors: ROBERTSON, G. S. M, CHADWICK, D. R, CONTRACTOR, H, JAMES, R. F. L, BELL, P. R. F, LONDON, N. J. M
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Separation - methods
Diabetes. Impaired glucose tolerance
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Humans
Hydrogen-Ion Concentration
Islets of Langerhans - cytology
Islets of Langerhans Transplantation
Medical sciences
Osmolar Concentration
Pancreas - cytology
Serum Albumin, Bovine
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Summary:The purification of large numbers of human pancreatic islets remains one of the limiting factors in islet transplantation. This paper describes and validates a method for accurately and reproducibly determining the density of islets and exocrine tissue in pancreatic digest on the basis of their isopycnic distribution on linear continuous density gradients. The use of this data to analyse and compare the purity of a standard 60% islet yield is described. The results obtained using such gradients will enable factors responsible for the variation in yield between pancreases to be determined and optimized, improving the results and reliability of islet purification.
ISSN:0940-5429
1432-5233
DOI:10.1007/bf00572864