Identification and characterization of a Mycoplasma synoviae 55,000-molecular-weight antigen associated with hemagglutination

Serological studies have shown that some antigenic determinants are conserved among several pathogenic Mycoplasma species, including Mycoplasma pneumoniae, M. genitalium, and M. gallisepticum. M. synoviae, an avian pathogen that shares certain morphological and biological features with the above-men...

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Bibliographic Details
Published in:Avian diseases 1993-10, Vol.37 (4), p.1097-1104
Main Authors: Morsy, M.A, Panangala, V.S, Hu, P.C
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Monoclonal - isolation & purification
ANTIGENE
ANTIGENOS
Antigens
Antigens, Bacterial - analysis
Antigens, Bacterial - isolation & purification
Antigens, Bacterial - physiology
Antigens, Surface - analysis
Antiserum
Chickens
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Epithelium
Epithelium - microbiology
Epithelium - ultrastructure
EPREUVE D'HEMAGGLUTINATION
Erythrocytes
Gels
Hemagglutination
IDENTIFICACION
IDENTIFICATION
Immunoblotting
Mice
Mice, Inbred BALB C - immunology
Microscopy, Immunoelectron
Molecular Weight
Mycoplasma
Mycoplasma - immunology
Mycoplasma - isolation & purification
Mycoplasma pneumoniae
MYCOPLASMA SYNOVIAE
Organ Culture Techniques
PRUEBAS DE HEMAGLUTINACION
Trachea - microbiology
Trachea - ultrastructure
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Summary:Serological studies have shown that some antigenic determinants are conserved among several pathogenic Mycoplasma species, including Mycoplasma pneumoniae, M. genitalium, and M. gallisepticum. M. synoviae, an avian pathogen that shares certain morphological and biological features with the above-mentioned mycoplasmas, was examined by the protein immunoblot procedure for its reactivity with hyperimmune rabbit antiserum specific for the major (190,000 molecular-weight [MV]) adhesion PI protein of M. pneumoniae. A single polypeptide of M. synoviae of approximately 55,000 MW was recognized by, the anti-PI antiserum. The 55,000-MW antigen was electroeluted following electrophoretic separation of M. synoviae polypeptides, and the eluted protein was used for immunization of mice for the production of monoclonal antibodies (MAbs) and polyclonal antiserum. immunoelectron microscopy with MAbs and gold-conjugated secondary antibodies showed that the 55,000-MW antigen was located at the cell surface and was more densely clustered around the bleb-like protuberance of the cell. Immuno-affinity-purified 55,000-MW antigen, as well as the antibodies produced against it, blocked the hemagglutination by M. synoviae
ISSN:0005-2086
1938-4351
DOI:10.2307/1591920