Physiological oxygen tension is relevant to MHC-1 expression, spontaneous transformation, and interferon response of in vitro aging murine fibroblasts

Working from the hypothesis that modest deviations from physiological oxygen tension will influence cell characteristics important for infections/immunity and tumor development, cells were studied at three oxygen tensions during in vitro aging. Primary mouse embryo fibroblasts were established and s...

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Bibliographic Details
Published in:Experimental gerontology 1993-11, Vol.28 (6), p.573-578
Main Authors: Ebbesen, Peter, Hager, Henrik, Aboagye-Mathiesen, George, Petersen, Peter M., Lützhøft, Jan, Villadsen, Jan A., Zdravkovic, Milan, Dalsgaard, Anne Mette, Zachar, Vladimir
Format: Article
Language:English
Subjects:
Ageing, cell death
Animals
Biological and medical sciences
Cell physiology
Cell Transformation, Neoplastic
Cells, Cultured
Cellular Senescence
Fibroblasts - physiology
Fundamental and applied biological sciences. Psychology
H-2 Antigens - analysis
in vitro aging
in vitro transformation
interferon
Interferons - biosynthesis
major histocompatibility complex
Mice
Mice, Inbred BALB C
Molecular and cellular biology
Oxygen - analysis
oxygen tension
primary culture
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Summary:Working from the hypothesis that modest deviations from physiological oxygen tension will influence cell characteristics important for infections/immunity and tumor development, cells were studied at three oxygen tensions during in vitro aging. Primary mouse embryo fibroblasts were established and subsequently passaged at 3, 6, and 18 kPa oxygen tension (6 representing normal tissue tension and 18 being the conventionally tension at in vitro cultures). The growth rate was slightly higher at 6 than 3 and 18 kPa. All cultures eventually stopped growing and subsequently transformed to nonmalignant cells with unlimited growth capacity. Cells kept at 3 kPa reached the highest number of cell doublings before crisis. Stimulation with PolyI:C resulted in detectable interferon response only at the high oxygen tension, and after transformation none of the cultures responded with interferon production. Expression of the major histocompatibility complex H-2K was elevated above and below physiological oxygen tension, indicating regulatory processes optimizing MHC expression at about physiological oxygen tension.
ISSN:0531-5565
1873-6815
DOI:10.1016/0531-5565(93)90046-G