Elevation of oil body integrity and emulsion stability by polyoleosins, multiple oleosin units joined in tandem head-to-tail fusions

We have successfully created polyoleosins by joining multiple oleosin units in tandem head-to-tail fusions. Constructs encoding recombinant proteins of 1, 3 and 6 oleosin repeats were purposely expressed both in planta and in Escherichia coli. Recombinant polyoleosins accumulated in the seed oil bod...

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Bibliographic Details
Published in:Plant biotechnology journal 2010-10, Vol.8 (8), p.912-927
Main Authors: Scott, Richard W, Winichayakul, Somrutai, Roldan, Marissa, Cookson, Ruth, Willingham, Melanie, Castle, Maria, Pueschel, Ringo, Peng, Chi-Chung, Tzen, Jason T.C, Roberts, Nicholas J
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis - metabolism
Biological and medical sciences
Biotechnology
Electrophoresis, Polyacrylamide Gel
emulsion
Escherichia coli - genetics
Escherichia coli - metabolism
freezing tolerance
Fundamental and applied biological sciences. Psychology
germination
Inclusion Bodies - metabolism
lipid bodies
oil body
oleosin
Plant Oils - metabolism
Plant Proteins - genetics
Plant Proteins - metabolism
Plants, Genetically Modified - genetics
Plants, Genetically Modified - metabolism
polyoleosin
recombinant protein
Sesamum - genetics
Sesamum - metabolism
transgenic
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Summary:We have successfully created polyoleosins by joining multiple oleosin units in tandem head-to-tail fusions. Constructs encoding recombinant proteins of 1, 3 and 6 oleosin repeats were purposely expressed both in planta and in Escherichia coli. Recombinant polyoleosins accumulated in the seed oil bodies of transgenic plants and in the inclusion bodies of E. coli. Although polyoleosin was estimated to only accumulate to
ISSN:1467-7644
1467-7652
DOI:10.1111/j.1467-7652.2010.00522.x