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Elevation of oil body integrity and emulsion stability by polyoleosins, multiple oleosin units joined in tandem head-to-tail fusions
We have successfully created polyoleosins by joining multiple oleosin units in tandem head-to-tail fusions. Constructs encoding recombinant proteins of 1, 3 and 6 oleosin repeats were purposely expressed both in planta and in Escherichia coli. Recombinant polyoleosins accumulated in the seed oil bod...
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| Published in: | Plant biotechnology journal 2010-10, Vol.8 (8), p.912-927 |
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| Main Authors: | , , , , , , , , , |
| Format: | Article |
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| container_end_page | 927 |
| container_issue | 8 |
| container_start_page | 912 |
| container_title | Plant biotechnology journal |
| container_volume | 8 |
| creator | Scott, Richard W Winichayakul, Somrutai Roldan, Marissa Cookson, Ruth Willingham, Melanie Castle, Maria Pueschel, Ringo Peng, Chi-Chung Tzen, Jason T.C Roberts, Nicholas J |
| description | We have successfully created polyoleosins by joining multiple oleosin units in tandem head-to-tail fusions. Constructs encoding recombinant proteins of 1, 3 and 6 oleosin repeats were purposely expressed both in planta and in Escherichia coli. Recombinant polyoleosins accumulated in the seed oil bodies of transgenic plants and in the inclusion bodies of E. coli. Although polyoleosin was estimated to only accumulate to |
| doi_str_mv | 10.1111/j.1467-7652.2010.00522.x |
| format | article |
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Constructs encoding recombinant proteins of 1, 3 and 6 oleosin repeats were purposely expressed both in planta and in Escherichia coli. Recombinant polyoleosins accumulated in the seed oil bodies of transgenic plants and in the inclusion bodies of E. coli. Although polyoleosin was estimated to only accumulate to <2% of the total oil body protein in planta, their presence increased the freezing tolerance of imbibed seeds as well as emulsion stability and structural integrity of purified oil bodies; these increases were greater with increasing oleosin repeat number. Interestingly, the hexameric form of polyoleosin also led to an observable delay in germination which could be overcome with the addition of external sucrose. Prokaryotically produced polyoleosin was purified and used to generate artificial oil bodies and the increase in structural integrity of artificial oil bodies-containing polyoleosin was found to mimic those produced in planta. We describe here the construction of polyoleosins, their purification from E. coli, and properties imparted on seeds as well as native and artificial oil bodies. A putative mechanism to account for these properties is also proposed.</description><identifier>ISSN: 1467-7644</identifier><identifier>EISSN: 1467-7652</identifier><identifier>DOI: 10.1111/j.1467-7652.2010.00522.x</identifier><identifier>PMID: 20444209</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Arabidopsis - genetics ; Arabidopsis - metabolism ; Biological and medical sciences ; Biotechnology ; Electrophoresis, Polyacrylamide Gel ; emulsion ; Escherichia coli - genetics ; Escherichia coli - metabolism ; freezing tolerance ; Fundamental and applied biological sciences. Psychology ; germination ; Inclusion Bodies - metabolism ; lipid bodies ; oil body ; oleosin ; Plant Oils - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; polyoleosin ; recombinant protein ; Sesamum - genetics ; Sesamum - metabolism ; transgenic</subject><ispartof>Plant biotechnology journal, 2010-10, Vol.8 (8), p.912-927</ispartof><rights>2010 The Authors. Plant Biotechnology Journal © 2010 Society for Experimental Biology and Blackwell Publishing Ltd</rights><rights>2015 INIST-CNRS</rights><rights>2010 The Authors. Plant Biotechnology Journal © 2010 Society for Experimental Biology and Blackwell Publishing Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4102-cf7eb4d477ca96255c49a99736ebe0e767bea086599d49a03ca61cd8b74803313</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1467-7652.2010.00522.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1467-7652.2010.00522.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,11562,27924,27925,46052,46476</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1467-7652.2010.00522.x$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23326061$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20444209$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Scott, Richard W</creatorcontrib><creatorcontrib>Winichayakul, Somrutai</creatorcontrib><creatorcontrib>Roldan, Marissa</creatorcontrib><creatorcontrib>Cookson, Ruth</creatorcontrib><creatorcontrib>Willingham, Melanie</creatorcontrib><creatorcontrib>Castle, Maria</creatorcontrib><creatorcontrib>Pueschel, Ringo</creatorcontrib><creatorcontrib>Peng, Chi-Chung</creatorcontrib><creatorcontrib>Tzen, Jason T.C</creatorcontrib><creatorcontrib>Roberts, Nicholas J</creatorcontrib><title>Elevation of oil body integrity and emulsion stability by polyoleosins, multiple oleosin units joined in tandem head-to-tail fusions</title><title>Plant biotechnology journal</title><addtitle>Plant Biotechnol J</addtitle><description>We have successfully created polyoleosins by joining multiple oleosin units in tandem head-to-tail fusions. Constructs encoding recombinant proteins of 1, 3 and 6 oleosin repeats were purposely expressed both in planta and in Escherichia coli. Recombinant polyoleosins accumulated in the seed oil bodies of transgenic plants and in the inclusion bodies of E. coli. Although polyoleosin was estimated to only accumulate to <2% of the total oil body protein in planta, their presence increased the freezing tolerance of imbibed seeds as well as emulsion stability and structural integrity of purified oil bodies; these increases were greater with increasing oleosin repeat number. Interestingly, the hexameric form of polyoleosin also led to an observable delay in germination which could be overcome with the addition of external sucrose. Prokaryotically produced polyoleosin was purified and used to generate artificial oil bodies and the increase in structural integrity of artificial oil bodies-containing polyoleosin was found to mimic those produced in planta. We describe here the construction of polyoleosins, their purification from E. coli, and properties imparted on seeds as well as native and artificial oil bodies. A putative mechanism to account for these properties is also proposed.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>emulsion</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>freezing tolerance</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>germination</subject><subject>Inclusion Bodies - metabolism</subject><subject>lipid bodies</subject><subject>oil body</subject><subject>oleosin</subject><subject>Plant Oils - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>polyoleosin</subject><subject>recombinant protein</subject><subject>Sesamum - genetics</subject><subject>Sesamum - metabolism</subject><subject>transgenic</subject><issn>1467-7644</issn><issn>1467-7652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNpFkU9v1DAQxS0EoqXwFcAXxIUsE9uxE4lLqfpPVAUJKqReLCeZgBcnXmIHNnc-OA67LL549OY3b6R5hNAcVnl6b9arXEiVKVmwFYOkAhSMrbYPyPGh8fBQC3FEnoSwBmC5LORjcsRACMGgOia_zx3-NNH6gfqOeuto7duZ2iHi19HGmZqhpdhPLixIiKa2bpHrmW68m71DH-wQXtOERLtxSPcSnQYbA117O2Cb_GhMTtjTb2jaLPosmrSrmxbb8JQ86owL-Gz_n5C7i_PPZ1fZzYfL67PTm6wRObCs6RTWohVKNaaSrCgaUZmqUlxijYBKqhoNlLKoqjZ1gDdG5k1b1kqUwHnOT8irne9m9D8mDFH3NjTonBnQT0EryWSpSikS-XxPTnWPrd6MtjfjrP8dLgEv94AJjXHdaIbGhv8c50yCXFa-3XG_rMP50M9BL0HqtV4y0kteeglS_w1Sb_XHd9epSOPZbtyGiNvDuBm_a6m4KvSX20t9ewXv7-8F6IvEv9jxnfHapASDvvuUjDnkZToMKP4H0fCq0w</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>Scott, Richard W</creator><creator>Winichayakul, Somrutai</creator><creator>Roldan, Marissa</creator><creator>Cookson, Ruth</creator><creator>Willingham, Melanie</creator><creator>Castle, Maria</creator><creator>Pueschel, Ringo</creator><creator>Peng, Chi-Chung</creator><creator>Tzen, Jason T.C</creator><creator>Roberts, Nicholas J</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201010</creationdate><title>Elevation of oil body integrity and emulsion stability by polyoleosins, multiple oleosin units joined in tandem head-to-tail fusions</title><author>Scott, Richard W ; Winichayakul, Somrutai ; Roldan, Marissa ; Cookson, Ruth ; Willingham, Melanie ; Castle, Maria ; Pueschel, Ringo ; Peng, Chi-Chung ; Tzen, Jason T.C ; Roberts, Nicholas J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4102-cf7eb4d477ca96255c49a99736ebe0e767bea086599d49a03ca61cd8b74803313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>emulsion</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>freezing tolerance</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>germination</topic><topic>Inclusion Bodies - metabolism</topic><topic>lipid bodies</topic><topic>oil body</topic><topic>oleosin</topic><topic>Plant Oils - metabolism</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>polyoleosin</topic><topic>recombinant protein</topic><topic>Sesamum - genetics</topic><topic>Sesamum - metabolism</topic><topic>transgenic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Scott, Richard W</creatorcontrib><creatorcontrib>Winichayakul, Somrutai</creatorcontrib><creatorcontrib>Roldan, Marissa</creatorcontrib><creatorcontrib>Cookson, Ruth</creatorcontrib><creatorcontrib>Willingham, Melanie</creatorcontrib><creatorcontrib>Castle, Maria</creatorcontrib><creatorcontrib>Pueschel, Ringo</creatorcontrib><creatorcontrib>Peng, Chi-Chung</creatorcontrib><creatorcontrib>Tzen, Jason T.C</creatorcontrib><creatorcontrib>Roberts, Nicholas J</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Plant biotechnology journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Scott, Richard W</au><au>Winichayakul, Somrutai</au><au>Roldan, Marissa</au><au>Cookson, Ruth</au><au>Willingham, Melanie</au><au>Castle, Maria</au><au>Pueschel, Ringo</au><au>Peng, Chi-Chung</au><au>Tzen, Jason T.C</au><au>Roberts, Nicholas J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Elevation of oil body integrity and emulsion stability by polyoleosins, multiple oleosin units joined in tandem head-to-tail fusions</atitle><jtitle>Plant biotechnology journal</jtitle><addtitle>Plant Biotechnol J</addtitle><date>2010-10</date><risdate>2010</risdate><volume>8</volume><issue>8</issue><spage>912</spage><epage>927</epage><pages>912-927</pages><issn>1467-7644</issn><eissn>1467-7652</eissn><abstract>We have successfully created polyoleosins by joining multiple oleosin units in tandem head-to-tail fusions. Constructs encoding recombinant proteins of 1, 3 and 6 oleosin repeats were purposely expressed both in planta and in Escherichia coli. Recombinant polyoleosins accumulated in the seed oil bodies of transgenic plants and in the inclusion bodies of E. coli. Although polyoleosin was estimated to only accumulate to <2% of the total oil body protein in planta, their presence increased the freezing tolerance of imbibed seeds as well as emulsion stability and structural integrity of purified oil bodies; these increases were greater with increasing oleosin repeat number. Interestingly, the hexameric form of polyoleosin also led to an observable delay in germination which could be overcome with the addition of external sucrose. Prokaryotically produced polyoleosin was purified and used to generate artificial oil bodies and the increase in structural integrity of artificial oil bodies-containing polyoleosin was found to mimic those produced in planta. We describe here the construction of polyoleosins, their purification from E. coli, and properties imparted on seeds as well as native and artificial oil bodies. A putative mechanism to account for these properties is also proposed.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>20444209</pmid><doi>10.1111/j.1467-7652.2010.00522.x</doi><tpages>16</tpages></addata></record> |
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| identifier | ISSN: 1467-7644 |
| ispartof | Plant biotechnology journal, 2010-10, Vol.8 (8), p.912-927 |
| issn | 1467-7644 1467-7652 |
| language | eng |
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| source | Wiley Open Access |
| subjects | Arabidopsis - genetics Arabidopsis - metabolism Biological and medical sciences Biotechnology Electrophoresis, Polyacrylamide Gel emulsion Escherichia coli - genetics Escherichia coli - metabolism freezing tolerance Fundamental and applied biological sciences. Psychology germination Inclusion Bodies - metabolism lipid bodies oil body oleosin Plant Oils - metabolism Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism polyoleosin recombinant protein Sesamum - genetics Sesamum - metabolism transgenic |
| title | Elevation of oil body integrity and emulsion stability by polyoleosins, multiple oleosin units joined in tandem head-to-tail fusions |
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