Automated video image morphometry of the corneal endothelium

The central corneal endothelium of 13 eyes in 13 subjects was visualized with a non-contact specular microscope. This report describes the computer-assisted morphometric analysis of enhanced digitized images, using a direct input by means of a frame grabber. The output consisted of mean cell area, c...

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Bibliographic Details
Published in:Documenta ophthalmologica 1993-01, Vol.85 (1), p.35-44
Main Authors: SIERTSEMA, J. V, LANDESZ, M, VAN DEN BROM, H, VAN RIJ, G
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Cell Count
Endothelium, Corneal - cytology
Female
Humans
Image Processing, Computer-Assisted - methods
Investigative techniques of ocular function and vision
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Microscopy - methods
Middle Aged
Reproducibility of Results
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Description
Summary:The central corneal endothelium of 13 eyes in 13 subjects was visualized with a non-contact specular microscope. This report describes the computer-assisted morphometric analysis of enhanced digitized images, using a direct input by means of a frame grabber. The output consisted of mean cell area, cell density, frequency distribution of the individual cell area, and cell polygonality. Results showed that the mean coefficient of variation of images analyzed three times consecutively was 0.95 percent. The cell analysis of three different images of the same subject was accurate, with a mean coefficient of variation of 4.2 percent.
ISSN:0012-4486
1573-2622
DOI:10.1007/BF01268098