Preparation for hapten help by glucan, muramyl dipeptide, and its L-ala-Glycerol-mycolate derivative

Previously, we reported that one of the factors that determines whether or not an animal will be prepared for hapten help after priming is the type of adjuvant used. The present work was undertaken, therefore, to determine which of a diverse variety of adjuvants or biological response modifiers woul...

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Bibliographic Details
Published in:Journal of leukocyte biology 1985-08, Vol.38 (2), p.317-325
Main Authors: Leech, Stephen H., Di Luzio, Nicholas R., Leclerc, Claude
Format: Article
Language:English
Subjects:
Acetylmuramyl-Alanyl-Isoglutamine - analogs & derivatives
Acetylmuramyl-Alanyl-Isoglutamine - immunology
Adjuvants, Immunologic
Analysis of the immune response. Humoral and cellular immunity
Animals
Antibody Formation
Biological and medical sciences
Cell interactions
Fundamental and applied biological sciences. Psychology
Fundamental immunology
glucan
Glucans - immunology
hapten help
Haptens - immunology
Immunobiology
Lymphocytes - immunology
macrophage activation
Macrophages - immunology
Mice
Mice, Inbred BALB C
muramyl dipeptide
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Summary:Previously, we reported that one of the factors that determines whether or not an animal will be prepared for hapten help after priming is the type of adjuvant used. The present work was undertaken, therefore, to determine which of a diverse variety of adjuvants or biological response modifiers would be effective. They included Freund's complete (CFA) and incomplete (FICA) adjuvants, particulate glucan, muramyl dipeptide (MDP), and its L‐ala‐glycerol‐mycolate derivative. Help by the azobenzenearsonate (ABA) hapten was measured as the augmentation of the anti‐bovine γ‐globulin (BGG) plaque‐forming cell (PFC) response to ABA‐BGG of mice that had been hapten‐primed with ABA conjugated to ovalbumin (OVA). The results showed that FICA was ineffective. MDP was effective but only if administered with FICA during hapten‐priming. MDP‐L‐ala‐glycerol‐mycolate was effective without any adjuvant but only within a narrow dose range. Particulate glucan was as effective as CFA in preparing mice for hapten help. As the macrophage is the primary cellular target of those biological response modifiers that were effective, we conclude that it plays an important role in the cellular interaction involved in the mediation of hapten help.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.38.2.317