Comparative evolution of endocytosis levels and of the cell surface area during the L929 cell cycle: A fluorescence study with TMA-DPH

• Published in: Biology of the cell 1993, Vol.79 (3), p.265-268
• Main Authors: Illinger, Dominique, Italiano, Liliane, Beck, Jean-Paul, Waltzinger, Caroline, Kuhry, Jean-Georges
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; cell cycle; Cell Cycle - physiology; Cell cycle, cell proliferation; Cell Membrane - physiology; Cell Membrane - ultrastructure; Cell physiology; cell synchronization; Diphenylhexatriene - analogs & derivatives; endocytosis; Endocytosis - physiology; Fluorescent Dyes; Fundamental and applied biological sciences. Psychology; Kinetics; L929 cells; Mice; Molecular and cellular biology; TMA-DPH
• ISSN: 0248-4900; 1768-322X
• DOI: 10.1016/0248-4900(93)90146-6

Summry— 1‐[4‐(trimethylamino)phenyl]‐6‐phenylhexa‐1,3,5‐triene (TMA‐DPH), a membrane fluorescence probe, interacts with living cells by instantaneous partition between the external medium and the plasma membrane, where it becomes fluorescent. The corresponding fluorescence intensity is then proportional to the cell surface. On the other hand, once incorporated into the plasma membrane, TMA‐DPH follows this membrane in the constitutive intracellular traffic and behaves as a monitor for endocytosis. Using this tool on L929 synchronized cells, we showed that the endocytosis levels after 30 min uptake of the probe increased from G1 to mitosis, when they abruptly decreased. The cell surface remained constant throughout the cell cycle, except at the beginning of mitosis when it almost doubled.