Rapid Detection of Human Cytomegalovirus in the Urine of Humans

"Immediate-early" cytomegalovirus-induced antigens are produced in infected cells within an hour of infection and "early" antigens appear at similar to 6 hr. The detection of these antigens, which appear both in productive and abortive CMV infection, can serve as a rapid diagnost...

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Bibliographic Details
Published in:The Journal of infectious diseases 1985-09, Vol.152 (3), p.631-633
Main Authors: Alpert, Gershon, Mazeron, Marie-Christine, Colimon, Ronald, Plotkin, Stanley
Format: Article
Language:English
Subjects:
Antibodies
Antibodies, Monoclonal
Antibodies, Viral
Antigens
Antigens, Viral - analysis
Biological and medical sciences
Bone Marrow Transplantation
Centrifugation
Child
Concise Communications
Cytomegalovirus
Cytomegalovirus - growth & development
Cytomegalovirus - immunology
Cytomegalovirus Infections - diagnosis
Cytomegalovirus Infections - urine
Fibroblasts
Flasks
Fluorescent Antibody Technique
Human viral diseases
Humans
Infant
Infectious diseases
Kidney Transplantation
Medical sciences
Monoclonal antibodies
Specimens
Tissue culture techniques
Urine
Urine - immunology
Urine - microbiology
Viral diseases
Viral Plaque Assay
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Description
Summary:"Immediate-early" cytomegalovirus-induced antigens are produced in infected cells within an hour of infection and "early" antigens appear at similar to 6 hr. The detection of these antigens, which appear both in productive and abortive CMV infection, can serve as a rapid diagnostic procedure eliminating the need to wait for the development of CPE. The present report describes the combined use of tissue culture, low-speed centrifugation, the E-13 monoclonal antibody, and biotin-avidin-amplified indirect fluorescent stain for rapid detection of CMV in urine. Recently, Gleaves et al. reported a similar method for rapid detection of CMV by using another monoclonal antibody and low-speed centrifugation.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/152.3.631