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Rapid Detection of Human Cytomegalovirus in the Urine of Humans
"Immediate-early" cytomegalovirus-induced antigens are produced in infected cells within an hour of infection and "early" antigens appear at similar to 6 hr. The detection of these antigens, which appear both in productive and abortive CMV infection, can serve as a rapid diagnost...
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| Published in: | The Journal of infectious diseases 1985-09, Vol.152 (3), p.631-633 |
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| Main Authors: | , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c329t-41d7fb0dfe128aaacda59b569d85c950f0c3fe727583a182c7fd87389a602d63 |
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| container_end_page | 633 |
| container_issue | 3 |
| container_start_page | 631 |
| container_title | The Journal of infectious diseases |
| container_volume | 152 |
| creator | Alpert, Gershon Mazeron, Marie-Christine Colimon, Ronald Plotkin, Stanley |
| description | "Immediate-early" cytomegalovirus-induced antigens are produced in infected cells within an hour of infection and "early" antigens appear at similar to 6 hr. The detection of these antigens, which appear both in productive and abortive CMV infection, can serve as a rapid diagnostic procedure eliminating the need to wait for the development of CPE. The present report describes the combined use of tissue culture, low-speed centrifugation, the E-13 monoclonal antibody, and biotin-avidin-amplified indirect fluorescent stain for rapid detection of CMV in urine. Recently, Gleaves et al. reported a similar method for rapid detection of CMV by using another monoclonal antibody and low-speed centrifugation. |
| doi_str_mv | 10.1093/infdis/152.3.631 |
| format | article |
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The detection of these antigens, which appear both in productive and abortive CMV infection, can serve as a rapid diagnostic procedure eliminating the need to wait for the development of CPE. The present report describes the combined use of tissue culture, low-speed centrifugation, the E-13 monoclonal antibody, and biotin-avidin-amplified indirect fluorescent stain for rapid detection of CMV in urine. 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The detection of these antigens, which appear both in productive and abortive CMV infection, can serve as a rapid diagnostic procedure eliminating the need to wait for the development of CPE. The present report describes the combined use of tissue culture, low-speed centrifugation, the E-13 monoclonal antibody, and biotin-avidin-amplified indirect fluorescent stain for rapid detection of CMV in urine. 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Mazeron, Marie-Christine ; Colimon, Ronald ; Plotkin, Stanley</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c329t-41d7fb0dfe128aaacda59b569d85c950f0c3fe727583a182c7fd87389a602d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Antibodies</topic><topic>Antibodies, Monoclonal</topic><topic>Antibodies, Viral</topic><topic>Antigens</topic><topic>Antigens, Viral - analysis</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow Transplantation</topic><topic>Centrifugation</topic><topic>Child</topic><topic>Concise Communications</topic><topic>Cytomegalovirus</topic><topic>Cytomegalovirus - growth & development</topic><topic>Cytomegalovirus - immunology</topic><topic>Cytomegalovirus Infections - diagnosis</topic><topic>Cytomegalovirus Infections - urine</topic><topic>Fibroblasts</topic><topic>Flasks</topic><topic>Fluorescent Antibody Technique</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infant</topic><topic>Infectious diseases</topic><topic>Kidney Transplantation</topic><topic>Medical sciences</topic><topic>Monoclonal antibodies</topic><topic>Specimens</topic><topic>Tissue culture techniques</topic><topic>Urine</topic><topic>Urine - immunology</topic><topic>Urine - microbiology</topic><topic>Viral diseases</topic><topic>Viral Plaque Assay</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alpert, Gershon</creatorcontrib><creatorcontrib>Mazeron, Marie-Christine</creatorcontrib><creatorcontrib>Colimon, Ronald</creatorcontrib><creatorcontrib>Plotkin, Stanley</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alpert, Gershon</au><au>Mazeron, Marie-Christine</au><au>Colimon, Ronald</au><au>Plotkin, Stanley</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid Detection of Human Cytomegalovirus in the Urine of Humans</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1985-09-01</date><risdate>1985</risdate><volume>152</volume><issue>3</issue><spage>631</spage><epage>633</epage><pages>631-633</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>"Immediate-early" cytomegalovirus-induced antigens are produced in infected cells within an hour of infection and "early" antigens appear at similar to 6 hr. The detection of these antigens, which appear both in productive and abortive CMV infection, can serve as a rapid diagnostic procedure eliminating the need to wait for the development of CPE. The present report describes the combined use of tissue culture, low-speed centrifugation, the E-13 monoclonal antibody, and biotin-avidin-amplified indirect fluorescent stain for rapid detection of CMV in urine. Recently, Gleaves et al. reported a similar method for rapid detection of CMV by using another monoclonal antibody and low-speed centrifugation.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>2993446</pmid><doi>10.1093/infdis/152.3.631</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1899 |
| ispartof | The Journal of infectious diseases, 1985-09, Vol.152 (3), p.631-633 |
| issn | 0022-1899 1537-6613 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76287606 |
| source | Oxford University Press Archive; JSTOR Journals and Primary Sources |
| subjects | Antibodies Antibodies, Monoclonal Antibodies, Viral Antigens Antigens, Viral - analysis Biological and medical sciences Bone Marrow Transplantation Centrifugation Child Concise Communications Cytomegalovirus Cytomegalovirus - growth & development Cytomegalovirus - immunology Cytomegalovirus Infections - diagnosis Cytomegalovirus Infections - urine Fibroblasts Flasks Fluorescent Antibody Technique Human viral diseases Humans Infant Infectious diseases Kidney Transplantation Medical sciences Monoclonal antibodies Specimens Tissue culture techniques Urine Urine - immunology Urine - microbiology Viral diseases Viral Plaque Assay |
| title | Rapid Detection of Human Cytomegalovirus in the Urine of Humans |
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