A prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormone

The cloned complementary DNA sequence encoding the human gonadotropin-releasing hormone (GnRH) precursor protein was used to construct an expression vector for the bacterial synthesis of the 56-amino acid GnRH-associated peptide (GAP). GAP was found to be a potent inhibitor of prolactin secretion an...

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Bibliographic Details
Published in:Nature (London) 1985-08, Vol.316 (6028), p.511-517
Main Authors: Nikolics, Károly, Seeburg, Peter H, Sz nyi, Éva, Mason, Anthony J, Ramachandran, J
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell physiology
Cells, Cultured
cloning
Cloning, Molecular
DNA - genetics
Follicle Stimulating Hormone - metabolism
Fundamental and applied biological sciences. Psychology
Gonadotropin-Releasing Hormone - genetics
Gonadotropin-Releasing Hormone - isolation & purification
Gonadotropin-Releasing Hormone - physiology
Hormonal regulation
Humanities and Social Sciences
Humans
Immunization
Luteinizing Hormone - metabolism
man
Molecular and cellular biology
multidisciplinary
Pituitary Gland, Anterior - metabolism
prolactin
Prolactin - antagonists & inhibitors
Prolactin - metabolism
Prolactin Release-Inhibiting Factors - physiology
Protein Precursors - genetics
Protein Precursors - physiology
Rabbits
Rats
Science
Secretory Rate
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Summary:The cloned complementary DNA sequence encoding the human gonadotropin-releasing hormone (GnRH) precursor protein was used to construct an expression vector for the bacterial synthesis of the 56-amino acid GnRH-associated peptide (GAP). GAP was found to be a potent inhibitor of prolactin secretion and to stimulate the release of gonadotropins in rat pituitary cell cultures. Active immunization with peptides corresponding to GAP sequences led to greatly increased prolactin secretion in rabbits.
ISSN:0028-0836
1476-4687
DOI:10.1038/316511a0