Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus

Comparative 16S rRNA sequence analysis has demonstrated that the genus Bacillus consists of at least five phyletic lines. rRNA group 3 bacilli of Ash, Farrow, Wallbanks and Collins (1991) comprising Bacillus polymyxa and close relatives is phylogenetically so removed from Bacillus subtilis, the type...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 1993, Vol.64 (3-4), p.253-260
Main Authors: Ash, C, Priest, F G, Collins, M D
Format: Article
Language:English
Subjects:
Bacillus - classification
Bacillus - genetics
Base Sequence
Classification - methods
DNA Primers
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction - methods
RNA, Ribosomal, 16S - genetics
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Summary:Comparative 16S rRNA sequence analysis has demonstrated that the genus Bacillus consists of at least five phyletic lines. rRNA group 3 bacilli of Ash, Farrow, Wallbanks and Collins (1991) comprising Bacillus polymyxa and close relatives is phylogenetically so removed from Bacillus subtilis, the type species of the genus and other aerobic, endospore-forming bacilli that they warrant reclassification in a new genus Paenibacillus. The genus Paenibacillus can be readily distinguished from other Bacillus groups using a battery of phenotypic characters and a highly specific gene probe based on 16S rRNA.
ISSN:0003-6072
1572-9699
DOI:10.1007/BF00873085