Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y

• Published in: International endodontic journal 2010-10, Vol.43 (10), p.835-842
• Main Authors: Killough, S. A., Lundy, F. T., Irwin, C. R.
• Format: Article
• Language: English
• Subjects: Blotting, Western; Cell Culture Techniques; Cell Proliferation - drug effects; Cells, Cultured; Coloring Agents; dental caries; Dental Caries - metabolism; Dental Pulp - cytology; Dental Pulp - drug effects; Dental Pulp - metabolism; Dentistry; Fibroblasts - drug effects; Fibroblasts - metabolism; human dental pulp fibroblasts; Humans; Interleukin-1beta - pharmacology; Membrane Proteins - analysis; Neuroimmunomodulation; neuropeptide Y; Neuropeptide Y - analysis; Neuropeptide Y - pharmacology; neuropeptide Y Y1 receptors; pulp biology; Receptors, Neuropeptide Y - analysis; Receptors, Neuropeptide Y - drug effects; Reverse Transcriptase Polymerase Chain Reaction; Substance P - pharmacology; Tetrazolium Salts; Thiazoles; Transforming Growth Factor beta - pharmacology
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/j.1365-2591.2010.01741.x

Killough SA, Lundy FT, Irwin CR. Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y. International Endodontic Journal, 43, 835–842, 2010. Aim  To investigate whether dental pulp fibroblasts express neuropeptide Y (NPY) and NPY‐Y1 in vitro and to determine the effects of the cytokines including interlukin‐1β (IL‐1β), TGF‐ β1, substance P and NPY on the expression of NPY Y1. Methodology  Three primary fibroblast cell strains were obtained from freshly extracted human third molar teeth. RT‐PCR was utilized to detect expression of NPY and mRNA expression. Membrane protein samples were isolated, and protein expression was determined by Western blotting. Radioimmunoassay was used to quantify NPY expression in healthy (n = 35) and carious (n = 39) whole pulp samples, and the student’s t‐test was used to test for statistical significance. In addition, the 3‐(4,5‐Dimethylthiazol,2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay was used to assay fibroblast cell growth. Results  mRNA transcripts were found in all three fibroblast cell populations with the cytokines having a stimulatory effect on its expression (P