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Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y
Killough SA, Lundy FT, Irwin CR. Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y. International Endodontic Journal, 43, 835–842, 2010. Aim To investigate whether dental pulp fibroblasts express neuropeptide Y (NPY) and NPY‐Y1 in vitro and to determine the effects o...
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| Published in: | International endodontic journal 2010-10, Vol.43 (10), p.835-842 |
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| creator | Killough, S. A. Lundy, F. T. Irwin, C. R. |
| description | Killough SA, Lundy FT, Irwin CR. Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y. International Endodontic Journal, 43, 835–842, 2010.
Aim To investigate whether dental pulp fibroblasts express neuropeptide Y (NPY) and NPY‐Y1 in vitro and to determine the effects of the cytokines including interlukin‐1β (IL‐1β), TGF‐ β1, substance P and NPY on the expression of NPY Y1.
Methodology Three primary fibroblast cell strains were obtained from freshly extracted human third molar teeth. RT‐PCR was utilized to detect expression of NPY and mRNA expression. Membrane protein samples were isolated, and protein expression was determined by Western blotting. Radioimmunoassay was used to quantify NPY expression in healthy (n = 35) and carious (n = 39) whole pulp samples, and the student’s t‐test was used to test for statistical significance. In addition, the 3‐(4,5‐Dimethylthiazol,2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay was used to assay fibroblast cell growth.
Results mRNA transcripts were found in all three fibroblast cell populations with the cytokines having a stimulatory effect on its expression (P |
| doi_str_mv | 10.1111/j.1365-2591.2010.01741.x |
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Aim To investigate whether dental pulp fibroblasts express neuropeptide Y (NPY) and NPY‐Y1 in vitro and to determine the effects of the cytokines including interlukin‐1β (IL‐1β), TGF‐ β1, substance P and NPY on the expression of NPY Y1.
Methodology Three primary fibroblast cell strains were obtained from freshly extracted human third molar teeth. RT‐PCR was utilized to detect expression of NPY and mRNA expression. Membrane protein samples were isolated, and protein expression was determined by Western blotting. Radioimmunoassay was used to quantify NPY expression in healthy (n = 35) and carious (n = 39) whole pulp samples, and the student’s t‐test was used to test for statistical significance. In addition, the 3‐(4,5‐Dimethylthiazol,2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay was used to assay fibroblast cell growth.
Results mRNA transcripts were found in all three fibroblast cell populations with the cytokines having a stimulatory effect on its expression (P < 0.05). NPY mRNA was not detected in the cell strains. NPY‐Y1 receptor protein expression was visualized by Western blotting, and there was no effect of IL‐1β or TGF‐ β1 on its expression. The mean concentration of NPY‐Ir determined by radioimmunoassay in non‐carious teeth was 19.40 ng g−1 (±17.03 SD) compared to 29.95 ng g−1 (±20.99 SD) in carious teeth (P < 0.05).
Conclusion Human dental pulp fibroblasts express, but do not synthesize, NPY, demonstrating that the fibroblast is a target cell for NPY. The effect of proinflammatory cytokines suggests that fibroblasts play a neuroimmunomodulatory role in the pulpal response to dental caries and injury.</description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/j.1365-2591.2010.01741.x</identifier><identifier>PMID: 20636351</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Blotting, Western ; Cell Culture Techniques ; Cell Proliferation - drug effects ; Cells, Cultured ; Coloring Agents ; dental caries ; Dental Caries - metabolism ; Dental Pulp - cytology ; Dental Pulp - drug effects ; Dental Pulp - metabolism ; Dentistry ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; human dental pulp fibroblasts ; Humans ; Interleukin-1beta - pharmacology ; Membrane Proteins - analysis ; Neuroimmunomodulation ; neuropeptide Y ; Neuropeptide Y - analysis ; Neuropeptide Y - pharmacology ; neuropeptide Y Y1 receptors ; pulp biology ; Receptors, Neuropeptide Y - analysis ; Receptors, Neuropeptide Y - drug effects ; Reverse Transcriptase Polymerase Chain Reaction ; Substance P - pharmacology ; Tetrazolium Salts ; Thiazoles ; Transforming Growth Factor beta - pharmacology</subject><ispartof>International endodontic journal, 2010-10, Vol.43 (10), p.835-842</ispartof><rights>2010 International Endodontic Journal</rights><rights>2010 International Endodontic Journal.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4061-3cebf46e0922356a227dd8e5e0b5dfff72e0af2cf50106392f4cc02c0c752e083</citedby><cites>FETCH-LOGICAL-c4061-3cebf46e0922356a227dd8e5e0b5dfff72e0af2cf50106392f4cc02c0c752e083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20636351$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Killough, S. A.</creatorcontrib><creatorcontrib>Lundy, F. T.</creatorcontrib><creatorcontrib>Irwin, C. R.</creatorcontrib><title>Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y</title><title>International endodontic journal</title><addtitle>Int Endod J</addtitle><description>Killough SA, Lundy FT, Irwin CR. Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y. International Endodontic Journal, 43, 835–842, 2010.
Aim To investigate whether dental pulp fibroblasts express neuropeptide Y (NPY) and NPY‐Y1 in vitro and to determine the effects of the cytokines including interlukin‐1β (IL‐1β), TGF‐ β1, substance P and NPY on the expression of NPY Y1.
Methodology Three primary fibroblast cell strains were obtained from freshly extracted human third molar teeth. RT‐PCR was utilized to detect expression of NPY and mRNA expression. Membrane protein samples were isolated, and protein expression was determined by Western blotting. Radioimmunoassay was used to quantify NPY expression in healthy (n = 35) and carious (n = 39) whole pulp samples, and the student’s t‐test was used to test for statistical significance. In addition, the 3‐(4,5‐Dimethylthiazol,2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay was used to assay fibroblast cell growth.
Results mRNA transcripts were found in all three fibroblast cell populations with the cytokines having a stimulatory effect on its expression (P < 0.05). NPY mRNA was not detected in the cell strains. NPY‐Y1 receptor protein expression was visualized by Western blotting, and there was no effect of IL‐1β or TGF‐ β1 on its expression. The mean concentration of NPY‐Ir determined by radioimmunoassay in non‐carious teeth was 19.40 ng g−1 (±17.03 SD) compared to 29.95 ng g−1 (±20.99 SD) in carious teeth (P < 0.05).
Conclusion Human dental pulp fibroblasts express, but do not synthesize, NPY, demonstrating that the fibroblast is a target cell for NPY. The effect of proinflammatory cytokines suggests that fibroblasts play a neuroimmunomodulatory role in the pulpal response to dental caries and injury.</description><subject>Blotting, Western</subject><subject>Cell Culture Techniques</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Coloring Agents</subject><subject>dental caries</subject><subject>Dental Caries - metabolism</subject><subject>Dental Pulp - cytology</subject><subject>Dental Pulp - drug effects</subject><subject>Dental Pulp - metabolism</subject><subject>Dentistry</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>human dental pulp fibroblasts</subject><subject>Humans</subject><subject>Interleukin-1beta - pharmacology</subject><subject>Membrane Proteins - analysis</subject><subject>Neuroimmunomodulation</subject><subject>neuropeptide Y</subject><subject>Neuropeptide Y - analysis</subject><subject>Neuropeptide Y - pharmacology</subject><subject>neuropeptide Y Y1 receptors</subject><subject>pulp biology</subject><subject>Receptors, Neuropeptide Y - analysis</subject><subject>Receptors, Neuropeptide Y - drug effects</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Substance P - pharmacology</subject><subject>Tetrazolium Salts</subject><subject>Thiazoles</subject><subject>Transforming Growth Factor beta - pharmacology</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqNkE1P7CAUhonR6Fz1Lxh2rjr3AAU6CxfGbzN-RmNckZYeko6daYU2jv_-0js6C1eygcN53kN4CKEMxiyuv7MxE0omXE7YmEO8BaZTNl5ukNG6sUlGwFKR8CyTO-RPCDMAkCDYNtnhoIQSko3IwykuurymbV-31FWFb4o6D12guGw9hkAX2PumxbarSqSv9JVRjzaWjadF39FF0_1A9siWy-uA-1_7Lnk-P3s6uUymdxdXJ8fTxKagWCIsFi5VCBPOhVQ557osM5QIhSydc5oj5I5bJ-P_lJhwl1oL3ILVMrYysUsOV3Nb37z3GDozr4LFus4X2PTBaCWYUinwSGYr0vomBI_OtL6a5_7TMDCDTzMzgzYzaDODT_Pfp1nG6MHXI30xx3Id_BYYgaMV8FHV-Pnrwebq7Ho4xXyyylehw-U6n_s3o7TQ0rzcXpj7Ry2mQt-aG_EPBd-S-w</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>Killough, S. A.</creator><creator>Lundy, F. T.</creator><creator>Irwin, C. R.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201010</creationdate><title>Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y</title><author>Killough, S. A. ; Lundy, F. T. ; Irwin, C. R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4061-3cebf46e0922356a227dd8e5e0b5dfff72e0af2cf50106392f4cc02c0c752e083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Blotting, Western</topic><topic>Cell Culture Techniques</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Coloring Agents</topic><topic>dental caries</topic><topic>Dental Caries - metabolism</topic><topic>Dental Pulp - cytology</topic><topic>Dental Pulp - drug effects</topic><topic>Dental Pulp - metabolism</topic><topic>Dentistry</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>human dental pulp fibroblasts</topic><topic>Humans</topic><topic>Interleukin-1beta - pharmacology</topic><topic>Membrane Proteins - analysis</topic><topic>Neuroimmunomodulation</topic><topic>neuropeptide Y</topic><topic>Neuropeptide Y - analysis</topic><topic>Neuropeptide Y - pharmacology</topic><topic>neuropeptide Y Y1 receptors</topic><topic>pulp biology</topic><topic>Receptors, Neuropeptide Y - analysis</topic><topic>Receptors, Neuropeptide Y - drug effects</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Substance P - pharmacology</topic><topic>Tetrazolium Salts</topic><topic>Thiazoles</topic><topic>Transforming Growth Factor beta - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Killough, S. A.</creatorcontrib><creatorcontrib>Lundy, F. T.</creatorcontrib><creatorcontrib>Irwin, C. R.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Killough, S. A.</au><au>Lundy, F. T.</au><au>Irwin, C. R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y</atitle><jtitle>International endodontic journal</jtitle><addtitle>Int Endod J</addtitle><date>2010-10</date><risdate>2010</risdate><volume>43</volume><issue>10</issue><spage>835</spage><epage>842</epage><pages>835-842</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract>Killough SA, Lundy FT, Irwin CR. Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y. International Endodontic Journal, 43, 835–842, 2010.
Aim To investigate whether dental pulp fibroblasts express neuropeptide Y (NPY) and NPY‐Y1 in vitro and to determine the effects of the cytokines including interlukin‐1β (IL‐1β), TGF‐ β1, substance P and NPY on the expression of NPY Y1.
Methodology Three primary fibroblast cell strains were obtained from freshly extracted human third molar teeth. RT‐PCR was utilized to detect expression of NPY and mRNA expression. Membrane protein samples were isolated, and protein expression was determined by Western blotting. Radioimmunoassay was used to quantify NPY expression in healthy (n = 35) and carious (n = 39) whole pulp samples, and the student’s t‐test was used to test for statistical significance. In addition, the 3‐(4,5‐Dimethylthiazol,2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay was used to assay fibroblast cell growth.
Results mRNA transcripts were found in all three fibroblast cell populations with the cytokines having a stimulatory effect on its expression (P < 0.05). NPY mRNA was not detected in the cell strains. NPY‐Y1 receptor protein expression was visualized by Western blotting, and there was no effect of IL‐1β or TGF‐ β1 on its expression. The mean concentration of NPY‐Ir determined by radioimmunoassay in non‐carious teeth was 19.40 ng g−1 (±17.03 SD) compared to 29.95 ng g−1 (±20.99 SD) in carious teeth (P < 0.05).
Conclusion Human dental pulp fibroblasts express, but do not synthesize, NPY, demonstrating that the fibroblast is a target cell for NPY. The effect of proinflammatory cytokines suggests that fibroblasts play a neuroimmunomodulatory role in the pulpal response to dental caries and injury.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20636351</pmid><doi>10.1111/j.1365-2591.2010.01741.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Blotting, Western Cell Culture Techniques Cell Proliferation - drug effects Cells, Cultured Coloring Agents dental caries Dental Caries - metabolism Dental Pulp - cytology Dental Pulp - drug effects Dental Pulp - metabolism Dentistry Fibroblasts - drug effects Fibroblasts - metabolism human dental pulp fibroblasts Humans Interleukin-1beta - pharmacology Membrane Proteins - analysis Neuroimmunomodulation neuropeptide Y Neuropeptide Y - analysis Neuropeptide Y - pharmacology neuropeptide Y Y1 receptors pulp biology Receptors, Neuropeptide Y - analysis Receptors, Neuropeptide Y - drug effects Reverse Transcriptase Polymerase Chain Reaction Substance P - pharmacology Tetrazolium Salts Thiazoles Transforming Growth Factor beta - pharmacology |
| title | Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y |
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