Effect of Ni²⁺ and Cd²⁺ ions on thermally induced conformational transitions in poly(dA)-poly(dT) system

Effects of Ni²⁺ and Cd²⁺ ions on thermally induced conformational transitions in the poly(dA)·poly(dT) polynucleotide duplex and poly(dA)·2poly(dT) triplex under near physiological ionic conditions were studied by measurement of UV absorption melting curves and static light scattering intensity. The...

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Bibliographic Details
Published in:Biometals 2010-12, Vol.23 (6), p.1191-1201
Main Authors: Zozulya, Victor N, Ryazanova, Olga A, Zhigalova, Nataliya N, Blagoi, Yurii P
Format: Article
Language:English
Subjects:
Aggregation of poly(dA)
Biochemistry
Biomedical and Life Sciences
Cadmium
Cadmium Chloride - pharmacology
Cations, Divalent - pharmacology
Cd
Cell Biology
Conformational transitions
Ions
Life Sciences
Light
Light scattering
Medicine/Public Health
Metal concentrations
Microbiology
Ni
Nickel
Nickel - pharmacology
Nucleic Acid Conformation - drug effects
Nucleic Acid Denaturation - drug effects
Pharmacology/Toxicology
Plant Physiology
Poly dA-dT - chemistry
Poly(dA)-poly(dT) system
Polydeoxyribonucleotides - chemistry
Polymers
Spectrophotometry, Ultraviolet
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Summary:Effects of Ni²⁺ and Cd²⁺ ions on thermally induced conformational transitions in the poly(dA)·poly(dT) polynucleotide duplex and poly(dA)·2poly(dT) triplex under near physiological ionic conditions were studied by measurement of UV absorption melting curves and static light scattering intensity. The diagrams of conformational transitions in poly(dA)-poly(dT)-Me²⁺ systems were plotted. An aggregation in these polynucleotide systems arises at certain values of the metal ions concentration and the temperature after the polymer dissociation into single strands. The phenomenon is conditioned by the aggregation of poly(dA) via the interstrand cross-linking by the dication bridges. Unlike Ni²⁺, Cd²⁺ induces formation of very stable aggregates which did not disintegrate even upon cooling up to room temperature.
ISSN:0966-0844
1572-8773
DOI:10.1007/s10534-010-9369-2